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Editors

B.C.Harinath

M.V.R. Reddy

Contents 

ELISA For Immunodiagnosis and Immunomonitoring of Tropical Diseases  

Laboratory Protocols

1. Preparation of CAM sticks      

2. Preparation and determination of optimal dilutions of Enzyme Conjugates

3. Stick ELISA (indirect) Detection of tuberculous antibody           

4. Plate peroxidase ELI SA (inhibition) Detection of filarial antigen   

5.Ammonium sulphate precipitation Isolation of immunoglobulin rich fraction      

6. Dialysis and ultra membrane filtration

7. Ion exchange chromatography   Isolation of IgG from immunoglobulins

8. Freeze drying (1yophylisation)

9. Gel filtration chromatography Separation of Circulating Filarial Antigen (CFA2) from plasma

10. SDS Polyacrylamide gel electrophoresis (SDS- PAGE)     

11. Coomassie blue staining

12. Silver staining

13. Western blot analysis 

14. Fast Protein Liquid Chromatography (FPLC)

15. Immunofluorescence

16. Double diffusion

17. Counter current immunoelectrophoresis (CIEP)

18. Isolation of DNA from B malayi microfilariae      

19. Polymerase Chain Reaction (PCR)

Appendix - I: Buffer, Reagents and Enzyme substrates

Appendix- II: Immunological Reagents

Appendix - Ill: Materials and Suppliers   

JBTDRC Reseacrh Product Kits:

SevaFilachek

SevaTB ELISA

Acknowledgement