The enzyme linked immunosorbant
assay (ELISA) is a heterogeneous enzyme immunoassay that uses solid phases e.g.
plastic tubes, beads, microplates, membranes such as cellulose acetate or cellulose
nitrate. Stick ELISA was developed with cellulose acetate membrane square fixed
onto a strip of used X-ray sheet. This is simple, economic and requires less
antigen or antibody.
Materials:
a. Cleaned (exposed) X-ray films.
b. Cellulose acetate membrane.
c.Cyclohexanone.
d. Stick fast
Method:
1.Take 70 mm X 160 mm plastic
sheet or used and cleaned X-ray sheet. Cut 5 mm X 160 mm cellulose acetate
strip from commercially available 20 mm X160mm strip.
2.Apply adhesive consisting of
cyclohexanone and stick fast (1:1) at the end ofthe plastic sheet (5 mm
distance from the edge) with a glass rod.
3.Carefully overlay
the 5 x 160 mm
cellulose acetate strip on the
adhesive on plastic sheet with the
help of forceps.
4.Place a butter paper on
the cellulose acetate strip and
press so that the membrane gets fixed to plastic sheet firmly.
5.After 5 minutes cut the sheet
vertically at 5 mm intervals.