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Dr. Bhaskar. C. Harinath
Director, JBTDRC &
Coordinator, BIC & Arogyadham
Mahatma Gandhi Institute of Medical Sciences
Sevagram (Wardha) – 442102, Maharashtra, India
E-mail: bch@jbtdrc.org, bc_harinath@yahoo.com
Phone: (Off) +91 7152 284038
(Res.) (+91 7152) 260219
Telefax: (+9 17152) 284038Professor Bhaskar C. Harinath presently Director, JBTDRC and Honorary Professor in Biochemistry, after completing Ph.D. and Post doctoral study in United States under Fulbright programme, joined the first rural Medical Institute, Mahatma Gandhi Institute of Medical Sciences at Sevagram in 1970 which was just started as Gandhi Centenary Project in 1969, to establish the Department of Biochemistry. During the last forty two years, in addition to teaching and hospital biochemistry work, the department was developed into an advanced centre for Tropical Disease Research in particular on filaria and tuberculosis with all the sophisticated equipments with support from Unichem labs., USEFI, ICMR, CSIR, DST, DBT and WHO/TDR resulting in establishment of Jamnalal Bajaj Tropical Disease Research Centre on campus. The centre has been recognized by DBT for establishment of ‘Repository for filarial parasite and reagents’. Bioinformatics Centre one of the 146 centres in India was established and funded by DBT since 1999. Presently the research work at the Centre and Biochemistry is supported by grants from DBT, ICMR, DST and Kasturba Health Society.
The work on filaria and tuberculosis diagnostics has been well recognized in India and abroad. The most significant contribution has been in-house development of immunoscreening tests for filariasis and tuberculosis based on ELISA using purified W. bancrafti mf ES antigen and Tb ES antigens respectively. Mycobacterial ES-31 serine protease (SEVA TB ES-31 antigen) of diagnostic and drug target interest and biomarker was isolated and reported for the first time and has been patented. He has widely travelled in the world participating in International Seminars and Workshops at USA, Kenya, Australia, USSR, Malaysia, France, Singapore, Thailand, Brazil and China. He has guided 17 Ph.D and 8 M.D students with more than 200 research publications. The research work was recognized by Amrut Modi award -1986 (Unichem Labs), PA Kurup Endowment award – 1991 (SBC), Awadesh Saran Oration – 1992 (ACBI), Dr. B. C. Roy National award – 1992 (MCI) and Dhanvantari award – 2004 (Education Society, Wardha). AJ Thakur Award for Distinguished Clinical Biochemist of India by ACBI. Dr. Harinath was President of Indian Immunology Society (1990-92) and President of Association of Clinical Biochemists of India (1994-95). Dr. Harinath joined as Associate Professor in 1970, became Professor in 1976 and was Director - Professor during 1992-2002, Department of Biochemistry and was also Dean, MGIMS from March 93 to September 94, Silver Jubilee year of the Institute. He is presently Director, Jamnalal Bajaj Tropical Disease Research Centre established on the campus and Co-ordinator for Bioinformatics Centre (Supported by DBT) and Arogyadham (supported by KHS and CCRYN) with Yoga & Nature Cure Centre and Herbal Garden. In addition to research on filaria and tuberculosis, Dr. Harinath is actively involved as Editor of Sevamed, Quarterly Update on Infectious Disease Research and Traditional Medicine and developing Medical Informatics and Arogyadham on medical campus for research in Holistic Healthcare.
Brief Biodata
Qualification & Experience 1961 M.Sc. Biochemistry, Nagpur University 1968 Ph.D., Oklahoma State University, USA 1968-70 Post Doctoral fellow, Washington University School of Medicine, St.Louis 1976 Professor and Head, Department of Biochemistry 1992- 2002 Director-Professor and Head, Department of Biochemistry and
Jamnalal Bajaj Tropical Disease Research Centre, M.G.I.M.S.Oct 2002 - Director, JBTDRC & Co-ordinator, BIC & Arogyadham, Sevagram Awards / Recognition 1964
Fullbright Travel grant. 1971-76 UGC National Associate. Dec. 1977-Mar.88 WHO Visiting Scientist. 1986 Amrut Mody award for research in Basic Medical Sciences. 1984-88 Identified for filarial research under Indo-US STI programme. 1991 P A Kurup Endowment Award for Bio Medical Research
(Society of Biological Chemists of India).1990-92 President, Indian Immunology Society. 1992 Awadesh Saran Memorial Oration,(Association of Clinical Biochemists of India). 1992 Dr. B.C.Roy National Award for development of Speciality, (Medical Council of India). 1994 Indian Council of Management Executives, Samajshri award-Order of Merit for medicine. 1994-95 President, Association of Clinical Biochemists of India 1994 Felicitated as devoted teacher on Teacher's day by Lions Club of Wardha. 1997-2001 Member, WHO Expert Advisory Panel on Health Lab. Services. 1995-2001 Member, Executive Council, Shri Venkateshwara Institute of Medical Sciences, Tirupati. 1997 Biodata included in the Marquis Who's Who in the World, 14th Ed. 1998 AACC International Travel grant recipient for presenting research paper in Golden Jubilee Conference of AACC, Chicago. 2004 Dhanvantari Award, JM Education Society, Wardha 2004 Adviser, Indian Association for Medical Informatics June 2008 Guest Speaker, III Brazilian Conference on Tuberculosis, Salvador Oct. 2008 S.C. Parija Oration (Indian Academy of Tropical Parasitology) Nov. 2009 ACBI – AJ Thakur Award for Distinguished Clinical Biochemist Mar. 2011 Guest Speaker, EPS Global Shanghai International TB Conference, Shanghai, China Jan. 2012 Prof. M.C. Nath Memorial Lecture, RTMNU, Nagpur Research Interests:
Research Publications >200 Students guided Ph.D. (17), MD (8) Patents awarded 1. 184510 dated June 2, 2024 for Process of isolation of Seva TB ES-31 protein antigen for TB diagnosis. 2. 224560 dated October 17, 2023 for process for the preparation of Brugia malayi microfilarial excretory-secretory (mf ES-22) glycoprotein.
Participation in International Seminars, WHO/TDR Workshop/Training Courses : Nairobi (1979), Sydney (1979), USSR (1981), Malaysia (1982), France (1983), USA (1977, 1985 & 1998, 2001, 03, 05, 07, 09, 11), Singapore (1986), Thailand (1995), Brazil (2008), China (2011). Membership in Academics & Societies:
Publications in Tuberculosis
Jena L, Kumar S, Harinath BC. MycoProtease-DB: Useful resource for Mycobacterium tuberculosis complex and nontuberculous mycobacterial proteases. Bioinformation 2012; 8(24):1240-1242. (PMID:23275726)
Jena L, Kumar S, Harinath BC. MTB-PCDB: Mycobacterium tuberculosis proteome comparison database. Bioinformation. 2011;6(3):131-133. (PMID:21584191)
Vinita Hutke, Gauri Wankhade, Pranita J Waghmare, Harinath BC. ELISA protocol for rapid screening of potential anti tubercular drugs based on antigenic reactivity of mycobacterial ES-31 serine protease – a drug target supported by axenic culture of mycobacterium tuberculosis H37Ra strain in the presence of inhibitor”. Indian J Tuberc 2013 July;60(3):138-141.
Pranita J Waghmare, Gauri Wankhade, Anindita M, Kiran Wandile, CM Badole, BC Harinath. SEVA TB ELISA – Multi antigen and antibody assays for serodiagnosis of suspected cases of pulmonary and extra pulmonary tuberculosis in tertiary care hospital –A retrospective study. Asian Pacific Journal of Tropical Disease (2012):S827-S832.
Gauri Wankhade, Vinita Hutke, Pranita J Waghmare, Arup Kr. Misra, Sushil Kumar Varma, Harinath BC. Inhibitory effect of isoniazid and orlistat combination on mycobacterial ES-31 serine protease in vitro and on the growth of M.tb bacilli in axenic culture. Indian J Tuberc 2012;59:156-161.
G Wankhade, A Majumdar, P Kamble, Sajal De, BC Harinath. Multi-antigen and antibody assays (SEVA TB ELISA) for the diagnosis of Tuberculous pleural effusion. Indian J Tuberc 2012;59:78-82.
Singhal S, Majumdar A, Harinath BC, Kamble P. Use of inhouse developed M.TB ES antigen ELISA in diagnosis of tuberculosis. Indian Medical Gazette, July 2011; CXLV(7):279-282.
S Haldar, M Bose, P Chakrabarti, H F Daginawala, BC Harinath, R S Kashyap, S Kulkarni, A Majumdar, H K Prasad, C Rodrigues, S Singh, R Srivastava, GM Taori, M Varma-Basil, JS Tyagi. Improved Laboratory Diagnosis of Tuberculosis – The Indian Experience. Tuberculosis. Sept 2011;91(5):414-426.
A Majumdar, Gauri Wankhade, Pranita Kamble, Deepti Joshi, Harinath BC.A sensitive and specific ES-31 Antigen detection based fluorometric assay for confirmation of M.tb in cell culture’.Indian J of Experimental Biology, April 2011;49:304-306.
Anindita Majumdar, Gauri Wankhade, Pranit Kamble, Harinath BC. Effect of HIV protease inhibitors and orlistat on mycobacterial ES-31 serine protease, a potential drug target in Mycobacterium tuberculosis. Indian J of Tuberc 2011;58:4-10.
G Wankhade, A Majumdar, P Kamble, BC Harinath. Lipase activity of 31kDa Mycobacterial ES-31 serine protease. Biomedical Research 2011; 22 (1):45-48.
Harinath BC. Immunodiagnostics for Tuberculosis – Problems and progress. Indian J Tuberc July 2010;57:123-127. Editorial
Majumdar A, Kamble PD, Badole CM, Harinath BC. Prospective study of inhouse developed SEVA TB Peroxidase enzyme immunoassay for cocktail antigen and antibody in the diagnosis of Tuberculosis in suspected patients attending a tertiary care hospital located in rural area. Asian Pacific Jr of Tropical Medicine, May 2010; 3(5):.356-359.
Majumdar A, Kamble PD, Harinath BC. Detection of circulating free and immune-complexed antigen in pulmonary tuberculosis using cocktail of antibodies to Mycobacterium tuberculosis excretory secretory antigens by peroxidise enzyme immunoassay. Indian J Tuberc 2010;57: 67-74.
M Anindita, D Thamke, DK Mendiratta, BC Harinath. Potential of Mycobacterial Excretory Secretory Protein Antigens (SEVA TB ES-31, ES-43, EST-6 and ES-20) as Biomarkers to detect Mycobacterium Tuberculosis Bacilli. Indian Journal of Clinical Biochemistry, January 2010; 25(1):15-19.
M. Anindita, V. Upadhye, D. Thamke, D.K. Mendiratta, B.C. Harinath. Mycobacterial ES-31 serine protease – a biomarker for mycobacterium tuberculosis- a preliminary report. Indian J Tuberc 2009;56: 141-143.
Upadhye Vijay, Majumdar Anindita, Gomashe Ashok, Joshi Deepti, Gangane Nitin, Thamke Dipak, Mendiratta Deepak and Harinath, B. C. (2009). 'Inhibition of Mycobacterium tuberculosis secretory serine protease blocks bacterial multiplication both in axenic culture and in human macrophages', The Scandinavian Journal of Infectious Diseases. 2009, 41(8):569-576.,
Upadhye VJ, Gomase AV, Kumar S, Harinath BC. Isolation, characterization and kinetic studies on SEVA TB ES-31 antigen, a metallo-serine protease of interest in serodiagnosis. Indian J Tuberc 2009;56:22-29.
Majumdar A, Upadhye V, Harinath BC. A prospective study of inhouse developed SEVA TB ELISA using cocktail of antigen and their immunoglobulins in the diagnosis of the tuberculosis suspected patients in a tertiary care hospital located in rural area. Biomedical Research 2009; 20(1): 59-63.
Majumdar A, Upadhye V, Harinath BC. Peroxidase enzyme immunoassay for circulating SEVA TB ES-31 Antigen in pulmonary tuberculosis sera. Biomedical Research 2008; 19 (3): 201-206.
Niraj Shende, Sonika Gupta, Vijay Upadhye, Satish Kumar & Bhaskar C Harinath Detection of in vitro and in vivo released antigens of diagnostic interest in Mycobacterium tuberculosis by immunoblotting. Indian Journal of Experimental Biology. Vol. 46, January 2008, 22-26.
Shende N, Upadhye V, Kumar S, Harinath BC. A low molecular weight ES-20 protein released in vivo and in vitro with diagnostic potential in lymph node tuberculosis. Indian J Med Microbiol 2008;26:29-33
Vijay Upadhye, Santa-Saha (Roy), Niraj Shende, Satish Kumar, Bhaskar C. Harinath. Isolation of Mycobacterium tuberculosis protein antigens ES-31, ES-43 and EST- 6 of diagnostic interest from Tubercle Bacilli by affinity chromatography. Indian Journal of Experimental Biology 2007: 45: 599-602.
Shende N, Gupta S, Upadhye V, Kumar S and Harinath BC. Isolation and analysis of circulating tuberculous antigens in mycobacterium tuberculosis. Indian Journal of Tuberculosis. Indian J Tuberc 2007; 54:125-129.
Vijay Upadhye, Niraj Shende, Satish Kumar, B.C. Harinath. Detection of antibody and antigen in extrapulmonary tuberculosis patients sera using a cocktail of mycobacterial excretory secretory antigens and their antibodies. Biomedical Research 2007; 18(3):214-219.
Vijay Upadhye, Santa-Saha (Roy), Niraj Shende, Satish Kumar, Bhaskar C. Harinath. Isolation of Mycobacterium tuberculosis protein antigens ES-31, ES-43 and EST- 6 of diagnostic interest from Tubercle Bacilli by affinity chromatography. Indian Journal of Experimental Biology 2007: 45: 599-602.
Shende N, Upadhye V, Kumar S, Gangane N and Harinath BC. Study of M.tuberculosis ES-31 and ES-20 antigen levels in different pathogenic grades of lymph node tuberculosis. Int J Tuberc Lung Dis 2007; 11(2); 222-226.
Das AK, Banerjee S, Kumar S, Reddy MVR, Harinath BC. Immunoscreening of clinically suspected pulmonary and extrapulmonary tuberculosis cases using ES-31 and ES-41 antigens – Hospital case study. The Indian Practitioner, Dec. 2006; 59(12):801804.
Swati Bera, Niraj Shende, Satish Kumar and B.C. Harinath. Detection of Antigen and Antibody in Childhood Tuberculous Meningitis. Indian Journal of Pediatrics, Aug 2006; 73:675-679
Bhaskar Chinnaiah Harinath, Satish Kumar, Santa Saha Roy, Suvarna Hirudkar, Vijay Upadhye, Niraj Shende. A cocktail of affinity – purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis. Diag Microbiol Infect Dis 2006; 55(May):65-68.
Santa Saha-Roy, Niraj Shende, Satish Kumar and BC Harinath. Effectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production. Indian J of Experimental Biology, December 2005;43:1196-1198.
Sonika Gupta, Niraj Shende, Amerjeet Singh Bhatia, Satish Kumar and Bhaskar C Harinath. IgG subclass antibody response to mycobacterial serine protease at different stages of pulmonary tuberculosis. Med Sci Monit, 2005;11(2): CR585-588.
N. Shende, S. Gupta, AS Bhatia, S. Kumar, BC Harinath. Detection of free and immune complexed serine protease and its antibody in patients of tuberculosis with and without HIV co-infection. Int. J of Tuberc. Lung Disease 2005; 9(8): 915-919.
Gupta S, Shende N, Kumar S, Harinath BC Detection of antibodies to a cocktail of mycobacterial excretory-secretory antigens in tuberculosis by ELISA and Immunoblotting. Current Science June 2005;88(11):1825-1827.
S. Bhatia, Sonika Gupta, Neeraj Shende, Satish Kumar and B.C. Harinath. Serodiagnosis of Childhood Tuberculosis by ELISA. Indian J of Pediatr2005;16(1);23-2.
Sonika Gupta, Niraj Shende, Satish Kumar and B.C. Harinath. Isolation of excretory secretary protein 6 kDa antigen (ES-6) and its seroreactivity in patients with different stages of pulmonary tuberculosis and healthy house hold contacts. Biomedical Research 2005; 16(1);23-27
Shende N, Gupta S, Kumar S, Harinath BC. Levels of antibody, free antigen and immune complexed antigen by ELISA in different grades of sputum positive patients of pulmonary tuberculosis. Indian J of Pathology and Microbiology July 2004; 47(3): 438-440.
Gupta S, Shende N, Kumar S, Harinath BC. Antibody response to M.tb H37Ra excretory secretory ES-43 and ES-31 antigens at different stages of pulmonary tuberculosis. Biomedical Research, 2004; 15(1):76-79.
Gupta S, Kumar S, Harinath BC. Mycobacterium tuberculosis excretory secretory (ES) protein antigens of interest in diagnosis, prognosis and prediction of disease development. J. MGIMS, 2004 Sept.; 9(ii): 16-21. Review.
E Raji Nair, S Banerjee, S Gupta, S Kumar, MVR Reddy & BC Harinath. Seroreactivity and characterization of 31 kDa M.tb. secretory protease of diagnostic interest in tuberculosis. Trends Clin Biochem Lab Medicine 2003;330-338.
Banerjee S, Gupta S, Shende N, Kumar S & Harinath BC. Serodiagnosis of tuberculosis using two ELISA system. Ind. J. Clin. Biochem 2003; 18(2): 48-53.
Bhatia AS, Kumar S & Harinath BC. Immunodiagnosis of tuberculosis: An update. Ind. J. Clin. Biochem 2003; 18(2):1-5.
S. Banerjee, S. Kumar & B.C. Harinath, Isolation and characterization of in vivo released 41 kDa mycobacterial antigen in pulmonary and bone and joint tuberculosis and its identification with H37Ra in vitro released antigen. Int. J. Tuberc Lung Dis 2003; 7(3): 278-283.
S. Banerjee, S. Gupta , N. Shende, S. Kumar & B.C. Harinath, Cocktail of ES-31 and ES-41 antigens for screening of pulmonary and extrapulmonary tuberculosis Biomedical Research 2002; 13(2/3): 135-137.
S Banerjee, S Gupta, Satish Kumar, A V Shrikhande, MVR Reddy & BC Harinath. Seroreactivity of 31 kDa and 41 kDa mycobacterial secretory proteins isolated from culture filtrate in extra pulmonary tuberculosis. Indian J Patho Microbiol 2003; 43(2): 261-264.
Sonika Gupta, Niraj shende, Swati Banerjee, Satish Kumar, MVR Reddy and BC Harinath. Analysis of SEVA TB ES-31 antigen specific immunoglobulins IgM, IgA and IgG in sera of sputum and culture positive pulmonary tuberculosis. Indian J. of Clin. Biochemistry. 2002 Jan; 17(1): 5-8.
Swati Banerjee, E Raji Nair, Satish Kumar, N Gangane, MVR Reddy & BC Harinath. Seroreactivity of two different antigenic protein fractions of M.tb H37Ra excretory secretory antigen in pulmonary and extra pulmonary tuberculosis. J Comm Dis. 2001;33(2): 63-71.
Swati Banerjee, E Raji Nair, Satish Kumar, MVR Reddy & BC Harinath. Assay of tubercular antibody, circulating free and immune complexed antigen in the diagnosis of pulmonary tuberculosis. Indian J Clin Biochem 2001;16(2):203-206.
E Raji Nair, S Banerjee, S Kumar, MVR Reddy & BC Harinath. Isolation of Mycobacterium tuberculosis 31 kDa antigen protein of diagnostic interest from culture filtrate using anti ES-31 antibody by affinity chromatography. Ind. J. Clin. Biochem. 2001; 16: 132-135.
E Raji Nair, Swati Banerjee, Satish Kumar, MVR Reddy & BC Harinath. Purification and characterization of a 31 kDa mycobacterial excretory-secretory antigenic protein with a diagnostic potential in pulmonary tuberculosis. Indian J Chest Dis Allied Sci 2001; 43:81-90.
Nair E. R, Banerjee S, Kumar S, Reddy M. V. R. and Harinath B. C. “Biochemical characterization and Seroreactivity of in vitro and in vivo release 31 kDa antigen protein of diagnostic interest in tuberculosis.” Proceedings of the first international symposium in Recent Advances in Molecular biology, Allergy and Immunology, Vadodara, 3-5th September, 2000, 208-218.
Nair E. R, Banerjee S, Kumar S, Reddy M. V. R. and Harinath B. C. “Isolation of M.tb 31 kDa antigen protein of diagnostic interest from culture filtrate using affinity purified ES-31 antibody by affinity chromatography.” Proceedings of the first international symposium on Recent Advances in Molecular biology, Allergy and immunology, Vadodara, 3-5th September, 2000, 204-207.
Nair ER, Banerjee S, Kumar S & Harinath BC. Isolation and characterization of a 31 kDa Mycobacterial antigen from tuberculous sera and its identification with in vitro released culture filtrate antigen of M.tb H37Ra Bacilli. Scandinavian Journal of Infectious Diseases 2000; 32: 551-556.
Pramanik J, Lodam AN, Badole CM, Reddy MVR, Patond KR and Harinath BC. Detection of tubercular antibody and antigen in sera of bone and joint tuberculosis. Ind. J. Clin. Biochem. 2000; 15 (1): 22–28.
Lodam AN, Reddy MVR and Harinath BC. Immunodiagnosis of pulmonary tuberculosis by concomitant detection of antigen and antibodies of excretory-secretory protein of Mycobacterium tuberculosis H37Ra. J. Biosci. 1998; 23 (1): 91-93.
Nair ER, Kumar S, Reddy MVR and Harinath BC. Mycobacterium tuberculosis H37 Ra ESAS-7 an excretory-secretary antigen fraction of immunodiagnosic potential in pulmonary tuberculosis; Ind. J. Clin. Biochem. 1998; 13 (2): 98-105.
Harinath BC, Satish kumar and Reddy MVR. In vitro released antigens in diagnosis and immunomonitoring of filaria and tuberculosis. Ind. J. Clin. Biochem. 1997; 12 (suppl): 8-12.
Lodam AN, Pramanik J, Reddy MVR and Harinath BC. Diagnostic potential of fractionated Mycobacterium tuberculosis H37Ra excretory-secretory (EST-DE1) antigen in pulmonary tuberculosis. Ind. J. Clin. Biochem. 1997; 12 (1): 71-77.
Paramanik J, Lodam AN, Reddy MVR, Narang P and Harinath BC. Increased yield of excretory-secretory antigen with thyroxine supplementation in in vitro culture of tubercle bacilli. Ind. J. tub. 1997; 44: 185-190.
Lodam AN, Reddy MVR, Narang P, Gupta OP and Harinath BC. Fractionation, analysis and diagnostic utility of Mycobacterium tuberculosis H37Ra excretory-secretory antigen in pulmonary tuberculosis. Ind. J. Biochem. Biophys. 1996; 33: 66-71.
Basak A, Sinha Choudhary S, Lodam A.N, Gupta OP, Narang P, and Harinath B C. Detection of IgG using Mycobacterium tuberculosis H37 Ra ES antigen in tuberculosis by Penicillinase stick ELISA. Proceedings of CSIR Golden Jubilee symposium on tropical diseases “Molecular Biology and control strategies”. 1994; 558.
Satish Kumar, Chenthamarakshan V, Reddy MVR, Narang P, Gupta OP and Harinath B C. Detection of tuberculous= IgG antibodies using Mycobacterium tuberculosis H37Ra excretory-secretory antigen and tuberculin purified protein derivative. Ind. J. Exptl Biol. 1994; 32: 163-167.
Bhaskar A, Pradan P, Chaturvedi P, Basak A, Lodam AN, Narang P, and Harinath BC. Immunodiagnosis of childhood pulmonary and extrapulmonary tuberculosis using mycobacterium tuberculosis ES antigen by penicillinase ELISA. Annals of Trop Pead. 1994; 14: 25-30.
Sood J, Gupta OP, Narang P, Cheirmaraj K, Reddy MVR and Harinath BC. Penicillinase ELISA for detection of tubercular antigen in tuberculosis. J. Com. Dis. 1991; 23 (3): 173-177.