MENINGITIS

( SEVAMED 2001)

(Diagnosis, Diagnostics, Immunodiagnosis, Immunodiagnostics, Pathogenesis,  Vaccines & Drugs)

 

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1360. Balganesh M.  Lalitha MK.  Nathaniel R. Rapid diagnosis of acute pyogenic meningitis by a combined PCR dot-blot assay. Molecular & Cellular Probes.  14(2):61-9, 2000 Apr.

Abstract

  A multiplex PCR was employed to amplify unique conserved sequences of DNA   from the pathogens Haemophilus influenzae, Neisseria meningitidis and   Streptococcus pneumoniae from cerebrospinal fluid samples of patients   suffering from acute pyogenic meningitis. The accurate identification of   the PCR amplified product was achieved by hybridizing dot-blots of the PCR   products to probes which were specific, biotinylated internal sequences of   the amplified target DNA. Detection of the hybrids was done in a colour   reaction using streptavidin-alkaline phosphatase conjugate and BCIP/NBT   substrates. The entire protocol took only 7 h for the correct   identification of the pathogen present in clinical samples of   cerebrospinal fluid. The sensitivity and specificity were >95%. Copyright   2000 Academic Press.

 

1361. No Abstract

1362.   Bisharda A.  Chowdhury R.  Puliyel JM. Evaluation of leukocyte esterase reagent strips for rapid diagnosis of pyogenic meningitis [letter]. Indian Pediatrics.   36(9):955-6, 1999 Sep.

 

1363.   Buttery JP.  Moxon ER. Designing meningitis vaccines. [Review] [31 refs] Journal of the Royal College of Physicians of London.  34(2):163-8, 2000  Mar-Apr.

Abstract

  Conjugate polysaccharide vaccines are a recent intervention to combat the   relative inability of young children to mount an effective immune response   against encapsulated bacteria, especially Haemophilus influenzae (Hib),   Neisseria meningitidis (Nm) and Streptococcus pneumoniae (Sp). These   organisms cause the majority of community acquired septicaemia and   meningitis in UK children. Their capsular polysaccharides, important   virulence factors in evading phagocytosis, are poorly immunogenic in young  children compared to adults. Conjugation, by covalent linking, of the   polysaccharide to an immunogenic protein, has been demonstrated for each   of these organisms to produce good antibody response to the   polysaccharide. Conjugate Hib vaccines have  proven effective in reducing   Hib meningitis and invasive disease in the countries thathave introduced   them. Pneumococcal conjugate vaccines have proven effective in preventing   invasive disease caused by serotypes contained in the vaccines. Efficacy   studies are awaited for meningococcal conjugate vaccines. [References: 31]  

 

1364. Chiu CH.  Tsai JR.  Ou JT.  Lin TY. Typhoid fever in children: a fourteen-year experience [see comments]. T`aiwan Erh k`o i Hs eh Hui Tsa Chih.  41(1):28-32, 2000 Jan-Feb.

Abstract

  From 1982 to 1995, 71 children admitted in our medical center were   diagnosed to have  typhoid fever by culture or serology. Of the 71   children, most (83%) were aged 5-15 years. These children usually   presented with fever and gastrointestinal symptoms, including abdominal   pain, diarrhea, nausea or vomiting, and constipation. Hepatosplenomegaly   was the most common physical sign observed and abdominal tenderness ranked   the second. Thrombocytopenia occurring in 9 patients (13%) was the most   common mode of complication. Other complications included intestinal   perforation (3%), rectal bleeding (3%), ascites or pleural effusion (4%),   and meningitis (1%). The incidence of complications tended to be higher   among children 5 years of age or older (p = 0.31). Most patients responded   well to appropriate antimicrobial therapies. There was no mortality.   Relapse was observed in two children, although both had received 10 days   of chloramphenicol therapy. The clinical isolates of Salmonella typhi were   susceptible in vitro to all the antibiotics tested, including    chloramphenicol, which,  however, showed a higher MIC90 level than other   drugs tested. In conclusion, there were age-specific differences of   typhoid fever in children in terms of the incidence and morbidity and   antibiotic resistance of S. typhi has not been a problem in this area at   least up to 1995.  

 

1365. Curry DJ.  Frim DM. Delayed repair of open depressed skull fracture.  Pediatric Neurosurgery.  31(6):294-7, 1999 Dec. 

Abstract

  INTRODUCTION: Elevation and repair of an open depressed skull fracture is   often thought of as an emergency procedure. Common indications for   emergent elevation of a depressed skull fracture have been dural tear,   seizure, gross contamination or mass effect from bone or a sizable   underlying intracerebral hematoma. As treatment of head injury moves   towards management of cerebral perfusion pressure (CPP) rather than   intracranial pressure (ICP), we sought a way to maximize CPP in the   initial treatment of head-injured patients with depressed skull fractures   that would eventually require surgery by delaying surgery, when possible,   until after the initial period of elevated ICP. METHODS: Over a 12-month   period, 7 patients (all male, ages 1-15 years) were admitted to our   institution with the diagnosis of open depressed skull fracture without   significant mass effect requiring urgent decompression. All had   significant head trauma with altered mental status and a Glasgow Coma   Score of 3-12. Patients were treated with antibiotic prophylaxis   (nafcillin, ceftriaxone, metronidazole), seizure prophylaxis (phenytoin)   and underwent CPP management in an intensive care unit setting as   indicated by intracranial pressure monitoring or clinical assessment.   Length of medical management of CPP ranged from 4 to 12 days. Upon   stabilization of CPP, patients were operated for repair of their dural,   bone and scalp injuries. RESULTS: All 7 patients treated in the above   manner suffered no ill effects from their delayed surgery: there was no   meningitis, no late seizures, and no cerebrospinal fluid leak.   Complications attributable to delay were not present at follow-up ranging   from 12 to 24 months. CONCLUSIONS: We have delayed surgery for repair of   open depressed skull fractures in order to maximize medical management of   CPP in the setting of acute trauma. Among other considerations, the risk   of intraoperative hypotension occurring at a time of acutely raised ICP   was avoided by this delay. We conclude that there is a role, in this   specifically defined subset of head trauma patients, for delayed surgical   repair of open depressed skull fractures. Copyright 2000 S. Karger AG,   Basel.

 

 

1366. No Abstract

1367.   Hortal M.  Camou T.  Palacio R.  Dibarboure H.  Garcia A. Ten-year review of invasive pneumococcal diseases in children and adults  from Uruguay: clinical spectrum, serotypes, and antimicrobial resistance. International Journal of Infectious Diseases.  4(2):91-5, 2000.

Abstract

  OBJECTIVES: Since 1987, the Reference Laboratory of the Ministry of Health   of Uruguay has been monitoring infections due to Streptococcus pneumoniae   in patients under 5 years of age, in those between 5 to 14 years of age,   and in adults. The  purpose of the present study was to retrospectively   analyze a 10-year collection of invasive S. pneumoniae isolates from   children 5 to 14 years of age and adults. METHODS: The Reference   Children's Hospital, Pasteur Hospital, and two private hospitals in   Montevideo as well as four hospitals located in other representative areas   of the country participated in the pneumococcal surveillance program.   Based on the information available at the Microbiology Department of the   Central Public Health Laboratory (demographic data, date and site of   isolate, and clinical diagnosis), all patients with an invasive   pneumococcal disease were recorded. Pneumonia was clinically and   radiologically diagnosed and etiology was assessed by isolation of S.   pneumoniae from blood or pleural fluid. All specimens were collected at   the Emergency Service. Capsular serotyping and antimicrobial   susceptibilities were determined for each isolate. RESULTS: During the   10-year period, 228 invasive S. pneumoniae were identified and included in   the study (blood, n = 129; cerebrospinal fluid [CSF], n = 73; pleural   fluid, n = 20; peritoneal fluid, n = 3; synovial fluid, n = 1; pericardic   fluid, n = 1; abscess, n = 1). The most frequent clinical presentations   were pneumonia (n = 71) and meningitis (n = 69). Thirty-five adults had an   underlying condition including, four with malignancies, four with lupus,   two with human immunodeficiency virus (HIV)-infected, and two patients in   hemodialysis among others. Eighteen of the 228 patients died (7.9%   fatality rate), but only four of these had an underlying condition. Eleven   fatal cases were attributable to meningitis (2 children, 9 and 11 years   old; 9 adults, mean age, 59 y). Four patients with pneumonia and three   with sepsis died, including a splenectomized woman. Nine different   capsular serotypes (1, 5, 7, 9, 12, 15, 19A, 20, and 23A) were identified   among the 18 fatal cases. Resistance to penicillin, generally combined   with  trimethoprim-sulfamethoxazole, fluctuated annually, not surpassing   10%. CONCLUSIONS: The study results indicated that 96% of the serotypes   involved in severe pneumococcal diseases were included in the 23-valent   vaccine and that S. pneumoniae resistance to penicillin was moderate.

 

1368. Jolles S.  Sewell WA.  Leighton C. Drug-induced aseptic meningitis: diagnosis and management. [Review] [162 refs] Drug Safety.  22(3):215-26, 2000 Mar.

Abstract

  Drug-induced aseptic meningitis (DIAM) has been reported as an uncommon   adverse reaction with numerous agents. It is a diagnosis of exclusion, and   clinical signs and CSF findings vary greatly. The body of evidence   regarding DIAM is largely in the form of anecdotal case reports and must   be interpreted carefully bearing this in mind. The major categories of   causative agents are nonsteroidal anti-inflammatory drugs, antimicrobials,   intravenous immunoglobulin, intrathecal agents, vaccines and a number of   other less frequently reported agents. There appears to be an association   between DIAM and connective tissue disease, particularly systemic lupus   erythematosus, and ibuprofen. There are 2 major proposed mechanisms for   DIAM. The first involves direct irritation of the meninges by intrathecal   administration of the drug, and the second involves immunological   hypersensitivity to the drug, most likely type III and type IV   hypersensitivity. Recognition and diagnosis of DIAM is important, as it is   treatable by withdrawal of the drug and recurrence is prevented. The   outcome of DIAM is generally good, usually without long term sequelae.   This article describes the case reports of DIAM in the current literature   and discusses the diagnosis and management of this rare complication.  

 

1369. No Abstract

1370.  Kamat DV.  Chakravorty BP. Comparative values of CSF-LDH isoenzymes in neurological disorders. Indian Journal of Medical Sciences.  53(1):1-6, 1999 Jan.

Abstract

  The present study was carried out to evaluate the usefulness of   Cerebrospinal fluid (CSF) Lactate dehydrogenase (LDH) isoenzymes in the   diagnosis in tuberculous meningitis (TBM), pyogenic meningitis (PM), viral   encephalitis (VE) and hydrocephalus (HC). A characteristic dominance of   isoenzymes in cerebrospinal fluid was observed: LDH4 in TBM while LDH3 in   PM. However, in VE and HC, LDH2 and LDH1 were dominant respectively. The   control subjects revealed the presence of isoenzymes LDH1 and LDH2 in very   low concentrations. Pattern of LDH isoenzymes in CSF may serve as a diagnostic tool to differentiate these neurological disorders.  

 

1371. Kanamalla US.  Ibarra RA.  Jinkins JR. Imaging of cranial meningitis and ventriculitis. [Review] [77 refs] Neuroimaging Clinics of North America.  10(2):309-31, 2000 May.  

Abstract

   A spectrum of infectious diseases may affect the cranial meninges.  Contrast-enhanced MR imaging and fluid-attenuated inversion-recovery MR   techniques are superior to contrast-enhanced computed tomography for   detecting and characterizing most pathologic meningeal conditions. In   patients with meningitis, a major role of medical imaging is to identify   potential complications such as hydrocephalus, extra-axial mass-forming  infectious collections, parenchymal infarcts related to arteritis,   ventriculitis, and associated underlying parenchymal abscesses or   granuloma formations. Early diagnosis of such complications may reduce the   rate of morbidity and mortality in this group of patients. 

 

1372. Kim KS. E. coli invasion of brain microvascular endothelial cells as a pathogenetic basis of meningitis. [Review] [14 refs] Sub-Cellular Biochemistry.  33:47-59, 2000. 

Abstract

  A major limitation to advances in prevention and therapy of bacterial   meningitis is our incomplete understanding of the pathogenesis of this   disease. Successful isolation and cultivation of BMEC, which constitute   the blood brain barrier, and the development of experimental hematogenous   meningitis animal model, which mimics closely the pathogenesis of human    meningitis, enabled us to dissect the pathogenetic mechanisms of bacterial   meningitis. We have shown for the first time using E. coli as a paradigm   the mechanisms of bacterial crossing of the blood-brain barrier into the   central nervous system. We have shown that invasion of BMEC is a   requirement for E. coli K1 crossing of the blood-brain barrier in vivo   (Prasadarao et al., 1996b; Huang et al., 1995). We have identified several   novel E. coli proteins (i.e., Ibe10, Ibe7, and Ibe23) contributing to   invasion of BMEC. We have also established a novel phenotype, i.e.,   invasion of BMEC, of a well known major E. coli protein, OmpA. In   addition, we have shown that some of these E. coli proteins (i.e., OmpA,   Ibe10) interact with novel endothelial receptors present on BMEC, not on   systemic vascular endothelial cells. Further understanding and   characterization of these E. coli-BMEC interactions should allow us to   develop novel strategies to prevent this serious infection. In addition,  the in vitro and in vivo models of the blood-brain barrier and the   information derived from our study should be beneficial to investigating   the pathogenesis of meningitis due to other organisms such as group B   streptococci, Listeria monocytogenes, Streptococcus pneumoniae and   Citrobacter. 

 

 

1374. Kumar S.  Puri V.  Mehndiratta MM.  Gupta S.  Bhutani A.  Sharma C.  Paradoxical response to antitubercular drugs. Indian Journal of Pediatrics.  62(6):695-701, 1995 Nov-Dec.

Abstract

  Seven patients with paradoxical response to antitubercular drugs are   reported. In three cases of intracranial tuberculomas, newer lesions   appeared and in two cases preexisting tuberculomas enlarged. In two cases   of tubercular meningitis, multiple tuberculomas appeared. All these cases   exhibited newer symptoms and CT/MBI revealed the paradoxical response to   antitubercular drugs. All responded to continued conservative therapy,   with addition of pyrazinamide.  

 

1375. Leung WC.  Tregoning D. Issues arising from two related cases of childhood tuberculous meningitis. Public Health.  114(1):57-9, 2000 Jan. 

Abstract

  Two cases of childhood meningitis occurred in the same school within two   weeks. The cases were cousins. The first case died and the aetiology was    confirmed as tuberculous following a gene probe of a culture from the   meningeal tissues at postmortem. The second case, with similar life   threatening clinical features compatible with tuberculous meningitis,   recovered. Seventy-seven contacts of the two cases among the immediate and   extended families were screened. The adult sources of the infection were   found to be two aunts of the second case, who were nurses working in the   same nursing home. A further 73 contacts of the adult cases were   identified. A total of eight close contacts to the aunts were given   chemoprophylaxis and three were treated for tuberculosis. Contact tracing   was also undertaken in the nursing home but no further cases were   identified. These two cases highlight the difficulties in making a rapid   diagnosis in tuberculous meningitis, the difficulties in handling media   and public concern with limited information, and deciding on the   appropriate level of contact tracing in special circumstances.

 

1376.  MacLennan JM.  Shackley F.  Heath PT.  Deeks JJ.  Flamank C.  Herbert M.  Griffiths H.  Hatzmann E.  Goilav C.  Moxon ER. Safety, immunogenicity, and induction of immunologic memory by a serogroup C meningococcal conjugate vaccine in infants: A randomized controlled trial [see comments].  JAMA.  283(21):2795-801, 2000 Jun 7.

Abstract

  CONTEXT: Neisseria meningitidis is a common cause of meningitis and   septicemia in infants worldwide. Whether a meningococcal C conjugate   vaccine protects infants against the serogroup C strain is unknown.   OBJECTIVES: To determine whether a meningococcal C conjugate vaccine is   safe and immunogenic and induces immunologic memory in infants. DESIGN:

  Single-center, double-blind, randomized controlled trial in 1995 and 1996.   SETTING: Community, Oxfordshire, England. PARTICIPANTS: One hundred   eighty-two healthy infants. INTERVENTIONS: Participants were randomly   assigned to receive vaccination with 0. 5-mL doses of 1 of 2 lots of   meningococcal C conjugate vaccine (groups 1 and 2; n=60 in each group) or   a hepatitis B control vaccine (group 3; n=62), administered with routine   immunizations at 2, 3, and 4 months of age. Approximately half of each   group received meningococcal C conjugate vaccine and half received plain   meningococcal polysaccharide vaccine (MPS) at 12 months of age. MAIN   OUTCOME MEASURES: Serum antibodies to meningococcal C polysaccharide,   assayed by enzyme-linked immunosorbent assay, and serum bactericidal   activity (SBA), at 2, 3, 4, 5, 12, and 13 months of age; local and   systemic reactions, recorded for 6 days after each vaccination, compared   by intervention group. RESULTS: Meningococcal C conjugate vaccine was well   tolerated. After 3 doses, children in groups 1 and 2 achieved   significantly higher meningococcal C IgG geometric mean concentrations (21   and 17 U/mL, respectively, vs 0.20 U/mL; P<.001) and SBA titers (629 and   420, respectively, vs 4.1; P<. 001) than controls. At 12 months, antibody   concentrations had decreased in all groups but remained significantly   higher in children vaccinated with meningococcal C conjugate vaccine (SBA,   24 and 16 in groups 1 and 2, respectively, vs 4.2 in group 3; P<.001).   Following vaccination with MPS at 12 months of age, SBA in the   meningococcal C conjugate vaccine group was significantly higher than in   controls (SBA, 789 vs 4.5; P<.001). CONCLUSIONS: Our data indicate that   meningococcal C conjugate vaccine is safe and immunogenic and results in   immunologic memory when given with other routinely administered vaccines   to infants at 2, 3, and 4 months of age. JAMA. 2000;283:2795-2801

 

1377. Mahadevan S. Advances in diagnosis of tuberculosis. [Review] [19 refs] Indian Journal of Pediatrics.  63(2):153-7, 1996 Mar-Apr.

Abstract

  The diagnosis of childhood tuberculosis is acknowledged to be an imprecise   process since bacteriological confirmation is available in only 30-40% of   cases. Newer developments in diagnosis of tuberculosis include use of   fluorescent stains for smears, newer systems for radiometric detection of   mycobacteria, rapid sensitivity testing using firefly bioluminescence,   liquid chromatographic analysis of mycolic  acids, immunodiagnostics for M.   tuberculosis specific antigens and the impact of molecular diagnostics   with amplification methods. The search for simple, reliable test for early   stages of the disease (in particular TB meningitis) still continues.   

 

1378.No Abstract

1379. Maltezou HC.  Spyridis P.  Kafetzis DA. Tuberculosis during infancy. International Journal of Tuberculosis & Lung Disease.  4(5):414-9, 2000  May.

Abstract

  SETTING: A worldwide re-emergence of tuberculosis has been observed during   the last decade. However, few studies of infants with tuberculosis appear   in the literature. OBJECTIVE: To describe tuberculosis during infancy.   DESIGN: The records of all infants diagnosed with tuberculosis at a   tertiary care hospital from 1982 to 1998 were reviewed. RESULTS:   Thirty-nine infants with a median age of 10 months were identified, 59% of   whom presented during the second half of the study period. Diagnoses   included endothoracic tuberculosis (33 patients), meningitis (3), miliary   tuberculosis (2) and cervical lymphadenitis (1). Reasons for medical   evaluation were the onset of symptoms (25 patients), contact investigation   (12) and tuberculin skin test screening (2). Common signs and symptoms   included fever (22 patients), cough (7), appetite loss (4) and   wheezing/rales (4). Chest X-ray revealed hilar adenopathy (22 patients),   infiltrates (16), atelectases (3) and miliary pattern (2). Cultures were   attempted in nine patients and were positive in seven. All patients   responded promptly to treatment. No complications or deaths occurred.   CONCLUSION: Tuberculosis in infants has been diagnosed increasingly during   the last decade. Endothoracic tuberculosis predominates. One third of the   patients were diagnosed due to contact investigation. As early diagnosis   and treatment appears to prevent complications and reduce mortality,   pediatricians should be alert for tuberculosis in an infant with an   atypical picture suggestive of infection.  

 

1380. O'Farrell R.  Thornton J.  Brennan P.  Brett F.  Cunningham AJ. Spinal cord infarction and tetraplegia--rare complications of  meningococcal meningitis.  British Journal of Anaesthesia.  84(4):514-7, 2000 Apr. 

Abstract

  A previously healthy 25-yr-old female developed flaccid areflexic   tetraplegia, with intact cranial nerve function, 36 h after the diagnosis   of bacterial meningitis. Polymerase chain reaction studies of   cerebrospinal fluid and blood were positive for Neisseria meningitidis,   serogroup B. Magnetic resonance of the cervicothoracic spine revealed   increased signal intensity and expansion in the lower medulla, upper   cervical cord and cerebellar tonsils. Neurosurgical consultation   recommended hyperventilation, dexamethasone and regular mannitol therapy   rather than decompressive intervention. The clinical course over the   following 12 days was complicated by the development of progressive   central nervous and multisystem organ failure with disseminated   intravascular coagulopathy. Autopsy revealed cerebral oedema with cystic   infarction extending from the medulla to the upper cervical cord and   cerebellar tonsils. Flaccid areflexic tetraplegia with spinal cord   infarction has not been reported following bacterial infection in an   adult. The clinical implications would suggest complete central nervous   system evaluation of patients recovering from meningococcal meningitis,   since spinal cord lesions, although uncommon, do occur. In those very rare   situations where a patient develops significant peripheral neurological   deficits, urgent magnetic resonance imaging is warranted, to rule out an   infective focus or an underlying anatomical anomaly.  

 

1381. Ozates M.  Ozkan U.  Kemaloglu S.  Hosoglu S.  Sari I. Spinal subdural tuberculous abscess. Spinal Cord.  38(1):56-8, 2000 Jan.

Abstract

  OBJECTIVES: Spinal subdural abscess is rare and only 48 cases have been   described to date. In this report, we present an additional spinal   subdural tuberculous abscess. METHOD: Tuberculous meningitis was diagnosed   with clinical and laboratory findings in a 45-year-old man. A spinal   subdural abscess was demonstrated using MRI. Presence of the abscess was   revealed by surgical intervention. The diagnosis was confirmed by   pathological examination. RESULTS: The patient had been treated for   tuberculous meningitis 2 years previously. The disease recurred when   anti-tuberculous therapy was prematurely discontinued. During the second   treatment, the patient also underwent a ventriculo-peritoneal shunt   operation for hydrocephalus. Dizziness and weakness of both legs developed   after the postoperative period. Spinal MRI showed a spinal subdural   abscess as a iso-intense mass with spinal cord in the T1 and T2 weighted   images, ring like enhancement and compression on the spinal cord at T3-T4   level. The patient underwent surgery and the abscess was drained. 

  CONCLUSION: Tuberculosis may cause a spinal subdural abscess and although   it is a rare disorder, when encountered MRI is very useful in the   diagnosis.

 

1382.  Peltola H. Worldwide Haemophilus influenzae type b disease at the beginning of the  21st century: global analysis of the disease burden 25 years after the use  of the polysaccharide vaccine and a decade after the advent of conjugates.  [Review] [191 refs] Clinical Microbiology Reviews.  13(2):302-17, 2000 Apr.

Abstract

  Vaccination against Haemophilus influenzae type b (Hib) diseases began a   quarter of a century ago with a polysaccharide vaccine; this vaccine was   followed by four different conjugates 10 years later. In this review, the   burden of global Hib disease is quantified following this 25-year period   of vaccine availability to determine the potential impact of conjugate   vaccines. This task was accomplished by analysis of data available in 10   languages in 75 geographical regions of over 50 countries. All severe Hib   diseases, not only meningitis, were characterized, and special attention   was paid to the most vulnerable age group, i.e., children aged 0 to 4   years. Prior to vaccination, the weighted worldwide incidence of   meningitis in patients younger than 5 years was 57/100,000, and for all   Hib diseases except nonbacteremic pneumonia, it was 71/100,000, indicating   357,000 and 445,000 cases per year, respectively. At least 108,500 of   these children died. For all age groups combined, there were 486,000 cases   of Hib disease, excluding pneumonia, with 114,200 deaths and probably an   equal number of sequelae per annum. If the figures for nonbacteremic   pneumonia are included, a conservative estimate is that over 2.2 million   cases of infection and 520,000 deaths from Hib disease occurred worldwide,   but the true numbers might have been greater. Despite these large numbers   and availability of safe and efficacious vaccines, only 38,000 cases   annually are prevented-a meager 8% or less than a 2% reduction in cases,   depending on whether nonbacteremic pneumonia is included in the   calculations. Although vaccination has had great success in some affluent   countries, the current level of activity has had a very small impact   globally. The use of conjugates, preferably with a reduced number of doses   and in combination with other vaccines or perhaps in fractional doses,   should be extended to less privileged countries, where most Hib disease   occurs. 

 

1383. Pemde HK.  Harish K.  Thawrani YP.  Shrivastava S.  Belapurkar KM. C-reactive protein in childhood meningitides. Indian Journal of Pediatrics.  63(1):73-7, 1996 Jan-Feb.

Abstract

  Utility of C-reactive protein (CRP) latex agglutination test in meningitis   was evaluated. Serum CRP test was positive in 100% cases of meningitic   groups and 53% cases of "no meningitis (NM)" group. Cerebrospinal fluid   (CSF) CRP test was positive in 100% cases of pyogenic meningitis, whereas   it was negative in 95% cases of tuberculous meningitis and 100% cases of   NM group. CSF CRP test showed 100% sensitivity and negative predictive   values, 95-100% specificity and 94-100% positive predictive values for   various inter-group differentiations. This study concluded that CSF CRP   positive cases should be considered as pyogenic meningitis unless proved  otherwise. Routine use of this simple, reliable and inexpensive test is   recommended for rapid diagnosis and differential diagnosis f meningitis. 

1384. Reddy TS.  Smith D.  Roy TM. Primary meningococcal pneumonia in elderly patients. American Journal of the Medical Sciences.  319(4):255-7, 2000 Apr.

Abstract

  Neisseria meningitidis infection in humans usually manifests as meningitis   and septicemia with skin manifestations. Infections of the respiratory   tract with N meningitidis have been documented in the past, but often this   organism is not routinely considered in the differential diagnosis of   pneumonia. The pathogenic role of N meningitidis in lower respiratory   tract infections may be underestimated because its isolation is difficult,   particularly when oropharyngeal flora are present. We profile 2 elderly   patients with primary meningococcal pneumonia to show the importance of   Gram stain and culture in early diagnosis. These modalities helped guide  treatment and prophylactic measures.

 

1385. Satishchandra P.  Nalini A.  Gourie-Devi M.  Khanna N.  Santosh V.  Ravi   V.  Desai A.  Chandramuki A.  Jayakumar PN.  Shankar SK.  Profile of neurologic disorders associated with HIV/AIDS from Bangalore, south India (1989-96). Indian Journal of Medical Research.  111:14-23, 2000 Jan. 

Abstract

  One hundred patients (95 males, 5 females, mean age at presentation 31.6   +/- 9.4 yr) with various neurological disorders associated with HIV   infection during 1989-1996 were evaluated at NIMHANS, Bangalore. Eighty   patients belonged to group I associated with opportunistic neuroinfections   and 20 to group II--non infectious neurological disorders. Cryptococcal   meningitis either alone (n = 31) or associated with tuberculous meningitis   (n = 6) was the most common (46.3%) followed by neurotuberculosis either   alone (n = 24) or with cerebral toxoplasmosis (n = 4) accounting for 35   per cent. Other opportunistic neuroinfections included cerebral   toxoplasmosis, herpes zoster, fulminant pyogenic meningitis and   neurosyphilis. Clinical characteristics, diagnostic clues, their   laboratory and radiological profiles and problems encountered in diagnosis   and management of these opportunistic infections are highlighted. In group   II (19 males and one female; mean age of 32.6 +/- 9.4 yr), two patients   had cortical dementia, three acute brain stem involvement, two epilepsy and one had features suggestive of progressive multifocal   leukoencephalopathy. Two patients of group I during follow up developed  cortical dementia. Six had peripheral nervous system involvement similar   to Guillain-Barre syndrome. Sixty six patients (63 of group I and 3 of   group II) progressed to AIDS, 33 patients from group I and one patient   from group II succumbed to the disease. With the rapid increase in the   incidence of HIV/AIDS and an increase in the neurological manifestations   of HIV/AIDS it is important to recognise the magnitude of the problem for   health planning in India.

 

1386. Scott TF. Cerebral herniation after lumbar puncture in sarcoid meningitis.  Clinical Neurology & Neurosurgery.  102(1):26-8, 2000 Mar.

Abstract

  A patient with chronic meningitis due to neurosarcoidosis became comatose   within minutes of a lumbar puncture and died 24 h later. The diagnosis of   neurosarcoidosis was made post mortem. Development of cerebral herniation   may have been exacerbated by lumbar puncture. It was proposed that   arachnoid villi dysfunction may have contributed to very high intracranial   pressures in this patient, since post mortem examination revealed   communication between the ventricles and outlet foramina of the fourth   ventricle, and that herniation was in part due to an acute pressure   differential caused by lumbar puncture.  

 

1387.   Seward RJ.  Towner KJ. Evaluation of a PCR-immunoassay technique for detection of Neisseria  meningitidis in cerebrospinal fluid and peripheral blood. Journal of Medical Microbiology.  49(5):451-6, 2000 May.

Abstract

  A multiplex PCR-immunoassay for the rapid diagnosis of bacterial   meningitis from cerebrospinal fluid (CSF) or peripheral blood was compared   with conventional microbiological techniques used in the routine   diagnostic laboratory. The multiplex PCR was designed to detect   simultaneously a universal conserved sequence coding for bacterial 16S   rRNA and the Neisseria meningitidis porA gene. The PCR-immunoassay had a   detection limit of (3-5) x 10(2) cfu/ml (equivalent to 1-3 cfu/PCR) with   spiked CSF or blood samples, compared with (3-5) x 10(3) cfu/ml for PCR   followed by conventional agarose gel electrophoresis for detection of PCR   products. Of 294 CSF samples from patients suspected on clinical grounds   of suffering from meningitis, the PCR-immunoassay detected bacterial DNA   in 29 CSF samples, 15 of which were also positive for N. meningitidis DNA.   The 29 positive CSF samples comprised 25 samples that were also reported   positive and four that were reported negative by conventional methodology;   the latter four were all positive for N. meningitidis by the PCR-immunoassay. Of 173 peripheral blood samples examined, the   PCR-immunoassay detected bacterial DNA in 18 samples, 14 of which were   also positive for N. meningitidis DNA. In comparison, only 10 samples were   reported positive for N. meningitidis by conventional methodology. All   negative PCR-immunoassay results correlated with those obtained by   conventional methodology for both CSF and blood samples. The sensitivity   and speed of the PCR-immunoassay system indicated that it could be used as   a routine diagnostic test for meningococcal meningitis, enabling a  diagnosis to be made within 4 h of receipt of the specimen.  

1388.  Stockhammer G.  Poewe W.  Burgstaller S.  Deisenhammer F.  Muigg A. Kiechl S.  Schmutzhard E.  Maier H.  Felber S.  Schumacher P.  Gunsilius  E.  Gastl G. Vascular endothelial growth factor in CSF: a biological marker for  carcinomatous meningitis. Neurology.  54(8):1670-6, 2000 Apr 25.

Abstract

  OBJECTIVE: To determine the value of vascular endothelial growth factor   (VEGF) in CSF as a marker for carcinomatous meningitis (CM). METHODS: The   concentration of VEGF was measured by ELISA in matched samples of CSF and   serum collected from 162 patients. These included patients with solid   tumors with CM (n = 11) or brain metastases without concomitant CM (n =   12), paraneoplastic neurologic syndromes (n = 4), viral (n = 15) and   bacterial (n = 20) meningitis, and a variety of non-neoplastic and   noninfectious neurologic diseases (n = 100). Using CSF/serum albumin   ratios, the VEGF index was calculated to estimate the proportion of   intrathecally produced VEGF. Immunohistochemical staining for VEGF was   performed in a brain metastasis from a mammary carcinoma associated with   CM. RESULTS: High VEGF levels (median 6,794.8 pg/mL) were found in CSF of   all patients with CM, whereas VEGF levels in matched sera were comparable   to other disease groups. In patients with CM, the concentration of VEGF in   CSF decreased significantly following antineoplastic treatment. In CSF   samples from patients with brain metastases without concomitant CM, VEGF   was not detectable. Median VEGF concentration in CSF from patients with   acute bacterial meningitis was 38.6 pg/mL, with only 9 of these 17   patients showing detectable VEGF levels in CSF. The VEGF indices in   patients with bacterial meningitis were significantly lower than in tumor   patients with CM (<22.8 versus >62.3), suggesting that the proportion of intrathecally produced VEGF is much higher in patients with CM as compared   with patients with bacterial meningitis. Patients without neoplastic or   infectious neurologic disorders consistently showed VEGF levels in CSF   below the assay detection limit of 25 pg/mL. Immunohistochemistry revealed   strong cytoplasmic staining for VEGF in a metastatic lesion from breast   cancer infiltrating the meninges. CONCLUSION: In patients with   carcinomatous meningitis, significant amounts of VEGF are released into   CSF. This study yields preliminary evidence that VEGF in CSF may be a   useful biologic marker for both the diagnosis and evaluation of treatment   response in carcinomatous meningitis.

 

1389. Terletskaia-Ladwig E.  Metzger C.  Schalasta G.  Enders G. Evaluation of enterovirus serological tests IgM-EIA and complement  fixation in patients with meningitis, confirmed by detection of enteroviral RNA by RT-PCR in cerebrospinal fluid. Journal of Medical Virology.  61(2):221-7, 2000 Jun. 

Abstract

  An enzyme immunoassay (EIA) for detection of anti-enterovirus IgM   antibodies was compared with complement fixation test in 43 patients with   confirmed enterovirus meningitis by RT-PCR of cerebrospinal fluids (CSF).   In 34% of patients with enterovirus meningitis, IgM antibodies could be   found, whereas complement fixation tests were positive in only 20%. The   specificity was determined with sera of 105 patients with non-enterovirus   meningitis. Specificity of IgM EIA and of complement fixation was 94% and   85%, respectively. In four patients with meningitis but without enterovirus detection in CSF, RT-PCR and virus isolation from stools were   positive. In three of these patients, IgM antibodies were detected, giving   a strong indication of an enterovirus-associated disease. Because of the   high specificity of IgM EIA, diagnosis of enterovirus-associated diseases   can be carried out in a single serum sample, whereas by complement   fixation tests, only fourfold increases in antibody titres in paired sera   indicate an acute infection. The application of IgM EIA is especially   important in cases of meningitis when CSF samples are not available and for diagnosis of enterovirus diseases with other clinical symptoms such as   fever, enteritis, and hand-foot-and-mouth disease. Copyright 2000   Wiley-Liss, Inc. 

1390.   Thomas RE. Preparing patients to travel abroad safely. Part 2: Updating vaccinations. Canadian Family Physician.  46:646-52, 655-6, 2000 Mar.

Abstract

  OBJECTIVE: To provide, for family physicians without access to a travel   clinic, evidence-based recommendations on vaccinating infants and   children, adults, pregnant women, and immunocompromised patients traveling   to non-Western countries. QUALITY OF EVIDENCE: Searches were undertaken of   MEDLINE from 1990 to November 1998 (372 articles); the Cochrane   Collaboration Library; publications of the National Action Committee on   Immunization and the Committee to Advise on Tropical Medicine and Travel   in Canada Communicable Disease Reports; the Canadian Immunization Guide;   and Laboratory Centre for Disease Control, United States Centres for   Disease Control, and World Health Organization websites. Evidence-based   statements, randomized controlled trials, systematic reviews, and   meta-analyses were selected. Vaccination recommendations are based on this   evidence. MAIN MESSAGE: Physicians should complete vaccination schedules   for children whose primary series is incomplete and vaccinate unvaccinated   adults. Hepatitis A is widespread, and travelers to areas where it is  endemic should be vaccinated. The elderly should be vaccinated against   influenza and pneumococcal disease. Pregnant women should receive vaccines   appropriate to their trimester. Immunocompromised patients should be   vaccinated, but BCG and live vaccines are contraindicated. Travelers to   areas where meningitis, typhoid, cholera, Japanese encephalitis, and   rabies are endemic should be vaccinated if they are likely to be exposed.   Those traveling to areas where tuberculosis is endemic should take   precautions and should have skin tests before traveling and 2 to 4 months   after return. CONCLUSIONS: Family physicians can administer all necessary   vaccinations. They can advise pregnant women and immunocompromised people   about the balance of risk of disease and benefits of vaccination.

 

1391. White AC Jr. Neurocysticercosis: updates on epidemiology, pathogenesis, diagnosis, and  management. [Review] [90 refs] Annual Review of Medicine.  51:187-206, 2000. 

Abstract

  Neurocysticercosis is now recognized as a common cause of neurologic   disease in developing countries and the United States. The pathogenesis   and clinical manifestations vary with the site of infection and   accompanying host response. Inactive infection should be treated   symptomatically. Active parenchymal infection results from an inflammatory   reaction to the degenerating cysticercus and will also respond to   symptomatic treatment. Controlled trials have not demonstrated a clinical   benefit for antiparasitic drugs. Ventricular neurocysticercosis often    causes  obstructive hydrocephalus. Surgical intervention, especially    cerebrospinal fluid diversion, is the key to management of hydrocephalus.   Shunt failure may be less frequent when patients are treated with   prednisone and/or antiparasitic drugs. Subarachnoid cysticercosis is   associated with arachnoiditis. The arachnoiditis may result in meningitis,   vasculitis with stroke, or hydrocephalus. Patients should be treated with   corticosteroids, antiparasitic drugs, and shunting if hydrocephalus is    present. 

 

1392. Wu HS.  Kolonoski P.  Chang YY.  Bermudez LE. Invasion of the brain and chronic central nervous system infection after systemic Mycobacterium avium complex infection in mice. Infection & Immunity.  68(5):2979-84, 2000 May.

Abstract

  Central nervous system (CNS) infections caused by nontuberculous   mycobacteria have been described previously, especially in patients with   AIDS. To investigate specific aspects of the pathogenesis of this entity,   C57BL bg(+)/bg(-) mice were infected intravenously with Mycobacterium   avium, and cultures of blood and brain as well as histopathology   examination of brain tissue were carried out at several time points up to   6 months after infection. Low-grade inflammatory changes with small   aggregates of lymphocytes and macrophages as well as perivascular cuffing   were seen early in the infection. A small number of bacteria could be   observed in the parenchyma of the choroid plexus. Six months after   infection, numerous bacteria were present within the foamy macrophage of   the granulomatous lesions along the ventricle and meninges. None of the   mice developed clinical signs of meningitis or encephalitis or even died   spontaneously during the period of observation. Use of CD18(-/-) knockout   mice indicated that transport of the bacterium within neutrophils or   monocytes into the brain is unlikely. Mild chronic CNS infection developed   in the mice during sustained systemic M. avium infection, similar to what   has been reported in most human cases.  

 

1798.  Andersen O. Myelitis. Current Opinion in Neurology.  13(3):311-6, 2000 Jun.

Abstract

  Acute transverse myelitis (ATM) with moderate symptomatology and smaller   multiple magnetic resonance imaging lesions is often caused by multiple   sclerosis. Severe ATM with extensive magnetic resonance imaging lesions   with or without associated meningitis often has a viral cause,   particularly in the younger age groups, whereas vascular disorders may   prevail among older patients. Previously, one had to rely on indirect   evidence such as viral serology or viral identification in throat washings  to confirm a diagnosis of myelitis. Thus, mycoplasma myelitis may occur   coincident with a mycoplasma pneumonia. Viral myelitis is now often   diagnosed by specific polymerase chain reaction of the cerebrospinal   fluid, for echovirus, Coxsackie virus, mumps virus, herpes simplex virus   or varicella-zoster virus, but an autoimmune component may still be   important. An anterior horn syndrome may be produced by the tick-borne   encephalomyelitis virus. Severe ATM may also be a postinfectious or  postvaccinal disorder [i.e. a partial acute disseminated encephalomyelitis   (ADEM)]. Neuromyelitis optica, a combination of severe myelitis and optic   neuritis, is often a manifestation of ADEM or systemic lupus   erythematosus. Many of these disorders are potentially treatable with   specific antiviral agents or immunosuppression. 'Idiopathic' ATM is   probably a consequence of inadequate examination and follow up. The   differential diagnoses-viral myelitis, multiple sclerosis, ADEM,   neuromyelitis optica, spinal arteriovenous malformation and   arteritis-should be considered and are usually identified by a rapid   diagnostic work-up, leaving few ATM cases undiagnosed.

 

1799.  Atobe JH.  Hirata MH.  Hoshino-Shimizu S.  Schmal MR.  Mamizuka EM. One-step heminested PCR for amplification of Neisseria meningitidis DNA in   cerebrospinal fluid.  Journal of Clinical Laboratory Analysis.  14(4):193-9, 2000. 

Abstract

  A one-step polymerase chain reaction (Heminested-PCR) was designed to   target the 16S rRNA fragment simultaneously using a set of primers for the   universal bacterial group and a Neisseria meningitidis species-specific   sequence for diagnostic purposes. The diagnostic features of the   Heminested-PCR were evaluated in the study of 168 cerebrospinal fluid   (CSF) specimens from 84 patients with a N. meningitidis infection,   meningitis caused by unrelated bacteria and other etiologies (57   patients), or suspicious cases (27 patients) with clinical symptoms of   bacterial meningitis but with negative results from bacteriological   procedures. About 90% of patients with bacterial meningitis, including   those suspicious cases, had prior antibiotic therapy. The sensitivity,   specificity, positive, and negative predictive values found in relation to   culture and/or microscopy were 91.7, 100, 100, 100, and 90.5%,   respectively. In patients suspected of having bacterial meningitis, the   Heminested-PCR revealed 51.9% (14 patients) positive for N. meningitidis   infection and 40.7% (11 patients) positive for unrelated bacterial   infections. The agreement of the Heminested-PCR with culture and/or   microscopy was high and ranked as almost perfect (kappa indices > 0.856),   in contrast to its agreement with other techniques. These findings speak   in favor of the molecular diagnosis of meningococcal meningitis in   patients who are culture- and/or microscopy-negative, due to their prior   antibiotic treatment. Copyright 2000 Wiley-Liss, Inc.

 

1800.  Auer M.  Pfister LA.  Leppert D.  Tauber MG.  Leib SL. Effects of clinically used antioxidants in experimental pneumococcal  meningitis. Journal of Infectious Diseases.  182(1):347-50, 2000 Jul.

Abstract

  Reactive oxygen intermediates mediate brain injury in bacterial   meningitis. Several antioxidant drugs are clinically available, including   N-acetylcysteine (NAC), deferoxamine (DFO), and trylizad-mesylate (TLM).   The present study evaluated whether these antioxidants are beneficial in a   model of pneumococcal meningitis. Eleven-day-old rats were infected   intracisternally with Streptococcus pneumoniae and randomized to   intraperitoneal treatment every 8 h with NAC (200 mg/kg), DFO (100 mg/kg),   TLM (10 mg/kg), or saline (250 microL). TLM-treated animals showed a  significantly reduced mortality compared with controls (P<.03). Meningitis  led to extensive cortical injury at 22+/-2.2 h after infection (median,   14. 6% of cortex; range, 0-61.1%). Injury was significantly (P<.01)   reduced to 1.1% (range, 0-34.6%) by NAC, to 2.3% (range, 0-19.6%) by DFO,   and to 0.2% (range, 0-36.9%) by TLM (the difference was not significant   among the 3 groups). None of the drugs reduced hippocampal injury. Thus,   several clinically used antioxidants reduced cortical injury in   experimental pneumococcal meningitis.  

 

 

1801.  Carrol ED.  Thomson AP.  Shears P.  Gray SJ.  Kaczmarski EB.  Hart CA.  Performance characteristics of the polymerase chain reaction assay to   confirm clinical meningococcal disease.  Archives of Disease in Childhood.  83(3):271-3, 2000 Sep.

Abstract

  BACKGROUND: Confirmation of clinical meningococcal disease (MCD) is   essential for management of patients, contacts, and outbreaks. Blood and   CSF cultures, the traditional gold standard diagnostic tests, have been   adversely affected by preadmission parenteral penicillin and fewer lumbar   punctures. Rapid, reliable serogroup determination without the need to   grow isolates could improve laboratory confirmation of MCD. AIMS: To   determine performance characteristics of the currently available   meningococcal polymerase chain reaction (PCR) assays in a clinical  setting. METHODS: Prospective study of 319 children presenting with a   suspected diagnosis of MCD (fever and a rash, or suspected bacterial   meningitis) over a 16 month period. RESULTS: A total of 166 (52% of all)   children had clinical MCD: diagnosis was confirmed microbiologically in   119 (72%) of these. Performance characteristics (sensitivity, specificity,   negative predictive value, positive predictive value) in confirmation of   clinical MCD were respectively (95% confidence interval): blood culture   31% (24-38%), 100%, 57% (49-65%), 100%; blood PCR 47% (39-55%), 100%, 65%  (58-73%), 100%; any test positive 72% (65-79%), 100%, 77% (70-84%), 100%.   CONCLUSIONS: Meningococcal DNA detection in blood or CSF by PCR is a   useful method of diagnosis of MCD. PCR of peripheral blood performs better   than blood culture. In a child with clinically suspected MCD, PCR assays,   bacterial antigen tests, and oropharyngeal swabbing for meningococcal   carriage should be performed in addition to blood or CSF culture, to   improve case confirmation.  

 

1802. DeRiemer K.  Soares EC.  Dias SM.  Cavalcante SC. Title   HIV testing among tuberculosis patients in the era of antiretroviral   therapy: a population-based study in Brazil.  International Journal of Tuberculosis & Lung Disease.  4(6):519-27, 2000   Jun.

Abstract

  SETTING: Rio de Janeiro, Brazil, a city with 29862 cases of tuberculosis   (TB) reported between January 1995 and June 1998. OBJECTIVES: To evaluate   the counseling and testing practices for human immunodeficiency virus   (HIV) infection among TB patients, and to identify the patient   characteristics associated with HIV screening as antiretroviral therapy   was introduced. DESIGN: Cross-sectional study of patients with TB who were   reported to the health department and who initiated anti-TB treatment. The   main outcome measure was screened versus not screened for HIV. RESULTS:   The proportion of TB patients who received HIV screening increased from   January 1995 through June 1998 (P < 0.001). Among young adults aged 20-49   years with TB, the independent predictors of HIV screening were a   diagnosis of both pulmonary and extrapulmonary TB (odds ratio [OR] = 2.4,   95% confidence interval [CI] 2.1-2.8); TB meningitis (OR = 13.5, 95%CI   6.5-31.5); disseminated TB (OR = 8.2, 95%CI 5.3-12.9); lymphatic TB (OR =   5.6, 95%CI 4.7-6.6); and male sex (OR = 1.4, 95%CI 1.3-1.6). Patients with   newly diagnosed TB who were women, lived in a low income neighborhood (OR   = 0.7, 95%CI, 0.6-0.7), and sought TB treatment in their own residential  neighborhood (OR = 0.3, 95%CI 0.3-0.4) were less likely to receive HIV   counseling and testing. CONCLUSION: Health care providers in Rio de   Janeiro selectively offered HIV counseling and testing to persons they   perceived to be at risk for HIV and those with advanced stages of TB. HIV  counseling and testing should be expanded and offered to all TB patients.

 

1803.  Drake R.  Dravitski J.  Voss L. Hearing in children after meningococcal meningitis. Journal of Paediatrics & Child Health.  36(3):240-3, 2000 Jun. 

Abstract

  OBJECTIVE: To review the outcome of sensorineural hearing loss in children   who have had confirmed meningococcal meningitis. METHODS: A retrospective   audit of children admitted to the Starship Children's Hospital with a   confirmed diagnosis of meningococcal meningitis during a 4-year period.   RESULTS: Sixty-five children had confirmed meningococcal meningitis.   Dexamethasone was administered according to recommended guidelines in 46   children (72%) while 12 children (19%) did not receive steroids at any   stage. All children were appropriately referred for hearing assessment.   Forty-nine children (75%) had their hearing tested and reliable   sensorineural evaluation was obtained in all but one case. Thirty-four   children (70%) were seen up to 6 weeks from discharge, 86% by 12 weeks.   Sixteen cases (25%) did not attend for audiological assessment.   Sensorineural hearing impairment was found in two children (4.2%).   CONCLUSIONS: Children with meningococcal meningitis were reliably referred   for audiological assessment but 39% either failed to attend for an outpatient hearing evaluation (25%) or had an unacceptable delay between   discharge and testing (14%). Of those reliably tested, two children (4.2%)   had significant sensorineural hearing loss.

 

1804. Eintracht S.  Silber E.  Sonnenberg P.  Koornhof HJ.  Saffer D. Analysis of adenosine deaminase isoenzyme-2 (ADA(2)) in cerebrospinal   fluid in the diagnosis of tuberculosis meningitis [letter]. Journal of Neurology, Neurosurgery & Psychiatry.  69(1):137-8, 2000 Jul.

 

1805.  Farinha NJ.  Razali KA.  Holzel H.  Morgan G.  Novelli VM.  Tuberculosis of the central nervous system in children: a 20-year survey.  Journal of Infection.  1(1):61-8, 2000 Jul.

Abstract

  OBJECTIVE: To review our experience of central nervous system (CNS)   tuberculosis at a major British paediatric tertiary referral centre,   following the introduction of CT Scan facilities. METHODS: This was a   retrospective case survey (prospective in nine patients) of patients   admitted to Great Ormond Street over a 20-year period (1977-1997), who   fulfilled criteria for a diagnosis of CNS tuberculosis. Data were  collected with regard to the clinical, laboratory and demographic   characteristics of patients, as well as results of radiological   investigations and data on clinical outcome. RESULTS: We identified 38   children with CNS tuberculosis: 23 with tuberculous meningitis (TBM), 10    with tuberculous meningitis and associated tuberculomas and five with   tuberculomas alone. The mean age of this group was 3.7 years, ranging from   8 months to 16 years. Only 14 (37%) were of Caucasian origin. A contact   source was identified in 18 patients (47%). Previous BCG had only been   given to six (16%). The main clinical symptoms and signs present on   admission were alteration in consciousness in 30 patients (79%), focal   neurological signs in 25 (66%) and fever in 25 (66%). Seizures were   observed in 20 patients (53%) and meningism in 18 (47%). Mycobacterium  tuberculosis was either cultured or identified by acid-fast stain from CSF   or brain tissue from 24 patients (63%). The Mantoux reaction was positive   in 17/33 (51%); abnormalities of the chest X-ray were found in 15 (40%).   Cranial CT scans of the patients presenting with TBM showed hydrocephalus   in 31 patients (94%), and basilar enhancement in 27 (93%) out of the 29   patients who received intravenous contrast. Anti-tuberculous therapy   administered varied according to current recommendations of the period;   concurrent steroids were given to 31 patients (82%). Neurosurgical procedures were required in the majority of patients with TBM, 25 (76%).   In five patients with TBM, new tuberculoma developed during treatment. The   overall mortality for our group of patients was 13%, whilst permanent   neurological sequelae were seen in 47% of the patients. None of the   patients who received BCG either died or had severe sequelae. Mortality   and morbidity rates were higher in the first 10 years of the study and   amongst those patients admitted in Stage III disease. CONCLUSIONS: CNS tuberculosis continues to be a condition which carries significant   morbidity and mortality. Early diagnosis and prompt initiation of   treatment are essential to improve the poor outcome. Copyright 2000 

 

1806. Garcia NS.  Castelo JS.  Ramos V.  Rezende GS.  Pereira FE. Frequency of myocarditis in cases of fatal meningococcal infection in   children: observations on 31 cases studied at autopsy. Revista Da Sociedade Brasileira de Medicina Tropical.  32(5):517-22, 1999  Sep-Oct.

Abstract

  The frequency of myocarditis associated with meningococcal disease in   children was reported only in two autopsied series (United States and   South Africa). Here we report the frequency of associated myocarditis in   31 children who died of meningococcal infection at Hospital Infantile N.S.   da Gloria in Vitoria, Espirito Santo State, Brazil. The diagnosis was   confirmed by isolation of Neisseria meningitidis. At least three sections   of fragments of both atria and ventricles were studied using the Dallas   Criteria for the morphologic diagnosis of myocarditis. The mean age was   47.6 +/- 39.8 months and the mean survival time after the onset of   symptoms was 46.1 +/- 26.5 h (12-112 h). Myocarditis was present in 13   (41.9%) patients, being of minimal severity in 11 cases and of moderate   severity in 2 cases. There were no cases with severe diffuse myocarditis.   The frequency of myocarditis was not influenced by sex, presence of   meningitis, survival time after the onset of symptoms or use of vasoactive   drugs. The frequency of myocarditis reported here was intermediate between   the values reported in the only two case series published in the   literature (57% in the United States and 27% in South Africa). Although   our data confirm the high frequency of myocarditis in meningococcal   disease, further investigations are necessary to elucidate the   contribution of myocarditis to myocardial dysfunction observed in cases of   meningococcal infection in children.  

 

1807. Hernandez Abanto SM.  Hirata MH.  Hirata RD.  Mamizuka EM.  Schmal M.oshino-Shimizu S.   Evaluation of Henes-PCR assay for Mycobacterium detection in different   clinical specimens from patients with or without tuberculosis-associated   HIV infection.  Journal of Clinical Laboratory Analysis.  14(5):238-45, 2000. 

Abstract

  The need for early diagnosis of tuberculosis, particularly in HIV-infected   patients, requires the development of diagnosis methods that have a high   sensitivity and specificity, as does the nucleic acid-based technology.   With the purpose of improving the detection of mycobacterium in different   clinical samples, we proposed and evaluated an assay based on nucleic   acid-amplification: heminested-PCR (Henes-PCR). The procedure was designed   to identify Mycobacterium spp., M. tuberculosis complex (MTC), and M.   avium complex (MAC), although it has the potential to include more primers   for the identification of other species. Analytical and clinical   evaluation of Henes-PCR was performed by analysis of reference strains and   356 clinical specimens from 246 patients with pulmonary and meningitis   tuberculosis and unrelated infections, including 142 HIV-infected   individuals. Ninety-three percent (199) positive and 100% (143) negative   results were obtained in specimens from patients with tuberculosis and   non-tuberculosis infection, respectively. The overall sensitivity of   Henes-PCR was 93.4%, specificity was 100%, positive and negative   predictive values were 100 and 91.1%, respectively. Sensitivity and   negative predictive value of Henes-PCR were significantly higher than   culture procedure for microscopy-negative specimens. Even though frequency   of HIV infection was higher in patients with tuberculosis, diagnostic   parameters of Henes-PCR were similar between HIV-positive and HIV-negative   patients. MTB was identified in 194 (98%) specimens while MAC was detected   in 5 (2%) specimens. These findings suggest that Henes-PCR is a useful   test for rapid detection of mycobacterium in clinically suspected cases of   tuberculosis with smear-negative results. Copyright 2000 Wiley-Liss, Inc.

 

1808.Hernandez-Albujar S.  Arribas JR.  Royo A.  Gonzalez-Garcia JJ.  Pena JM.  Vazquez JJ. Tuberculous radiculomyelitis complicating tuberculous meningitis: case   report and review. Clinical Infectious Diseases.  30(6):915-21, 2000 Jun. 

Abstract

  Tuberculous radiculomyelitis (TBRM) is a complication of tuberculous   meningitis (TBM), which has been reported rarely in the modern medical   literature. We describe a case of TBRM that developed in an human   immunodeficiency virus (HIV)-infected patient, despite prompt   antituberculous treatment. To our knowledge, this is the second case of   TBRM reported in an HIV-infected patient. We also review 74 previously   reported cases of TBRM. TBRM develops at various periods after TBM, even   in adequately treated patients after sterilization of the cerebrospinal   fluid (CSF). The most common symptoms are subacute paraparesis, radicular   pain, bladder disturbance, and subsequent paralysis. CSF evaluation   usually shows an active inflammatory response with a very high protein   level. MRI and CT scan are critical for diagnosis, revealing loculation   and obliteration of the subarachnoid space along with linear intradural   enhancement. As in other forms of paradoxical reactions to antituberculous   treatment, there is evidence that steroid treatment might have a   beneficial effect.  

 

1809.   Kao HT.  Huang YC.  Lin TY. Influenza A virus infection in infants.   Journal of Microbiology, Immunology & Infection.  33(2):105-8, 2000 Jun.

Abstract

  Influenza A virus causes a variety of respiratory and nonrespiratory   illness in children. The symptomatology varies with different age groups.   The purpose of this retrospective study was to define the clinical   characteristics of influenza A infection in Taiwanese infants. During the   period from December 1997 to February 1998, 37 febrile patients younger   than 1 year of age, including five newborns, were admitted to our hospital   due to suspicion of sepsis or meningitis. The medical records of these   patients were retrospectively evaluated. Influenza A virus was isolated   from the specimens of the throat swabs in all patients, whereas no   bacterial pathogen was detected. The most common clinical manifestations   of these infants were lower respiratory tract infections, including   pneumonia, bronchiolitis, and croup. There was no significant difference   between the clinical characteristics of infants younger than 3 months and   those aged from 3 months to 1 year. The mean duration of fever, peak of   body temperature, and duration of hospitalization were 3.41 (+/-1.86)   versus 4.4 (+/-2.02) days, 39.0 (+/-0.57) versus 39.9 (+/-0.63) oC,   4.9(+/-1.49) versus 6.3 (+/-3.7) days in infants younger than 3 months and   infants aged from 3 months to 1 year, respectively. The older infants aged   from 3 months to 1 year had a significantly higher peak body temperature   than the infants younger than 3 months (p < 0.05). Two patients with croup   had a more severe clinical course, however, the outcomes were good in all   patients. During an influenza A virus outbreak, influenza A infection   should be included in the differential diagnosis of infants with lower   respiratory tract infection.

 

1810. Kochar DK.  Kumawat BL.  Agarwal N.  Shubharakaran.  Aseri S.  Sharma BV.  Rastogi A. Meningoencephalitis in brucellosis.  Neurology India.  48(2):170-3, 2000 Jun. Abstract

  Human brucellosis, more specifically neurobrucellosis, is a less commonly   reported disease in India; although, animal brucellosis and seroprevalence   in specific areas is well reported. We are reporting 4 cases of   neurobrucellosis presenting as meningoencephalitis. Diagnosis was   confirmed by serological test and agglutination titre was > 1:320 in all   the patients. All these patients had close contact with animals and   history of raw milk ingestion was present in 3 cases. The aim of presenting these cases is to create awareness among physicians while   treating meningitis in persons, engaged in occupations related to   brucellosis or having a history of ingestion of raw milk or milk product.   

 

1811. Kozar MP.  Krahmer MT.  Fox A.  Gray BM.  Failure To detect muramic acid in normal rat tissues but detection in   cerebrospinal fluids from patients with Pneumococcal meningitis.   Infection & Immunity.  68(8):4688-98, 2000 Aug. 

Abstract

  Muramic acid serves as a marker for the presence of bacterial cell wall   debris in mammalian tissues. There have been a number of controversial and   sometimes conflicting results on assessing the levels of muramic acid in    health and disease. The present report is the first to use the   state-of-the art technique, gas chromatography-tandem mass spectrometry,   to identify and quantify the levels of muramic acid in tissues. Muramic   acid was not found in normal rat brain or spleen. However, when tissues   were spiked with muramic acid, it was readily identified. The detection   limit was <1 ng of muramic acid/100 mg (wet weight) of tissue. The levels   of muramic acid reported in diseased human spleen and spleen of arthritic   rats, previously injected with bacterial cell walls, were 100- to   1,000-fold higher. In the present study, muramic acid was also readily   detected in the cerebrospinal fluid of patients with pneumococcal  meningitis (6.8 to 3,900 ng of muramic acid/ml of cerebrospinal fluid). In   summary, there can be an enormous difference in the levels of muramic acid   found in different mammalian tissues and body fluids in health and   disease. This report could have great impact in future studies assessing   the role of bacterial cell wall remnants in the pathogenesis of certain   human inflammatory diseases.  

 

1813. Najioullah F.  Bosshard S.  Thouvenot D.  Boibieux A.  Menager B.  Biron   F.  Aymard M.  Lina B. Diagnosis and surveillance of herpes simplex virus infection of the central nervous system. Journal of Medical Virology.  61(4):468-73, 2000 Aug. 

Abstract

  Herpes simplex viruses (HSV) are responsible for neurological disorders   that require rapid diagnostic methods and specific antiviral therapy.   During 1997, 1431 cerebrospinal fluid samples (CSF) collected from 1339   patients with neurological disorder presentations were processed for HSV   detection. Eleven patients were positive for HSV, seven presenting with   encephalitis (6/7 due to HSV1) and 4 with aseptic meningitis (4/4 due to   HSV2). The incidence of HSV encephalitis was 2.33 cases / 10(6)   inhabitants/year. Among encephalitis (HSV encephalitis) cases, 1 patient   died due to the late implementation of antiviral therapy, and sequelae   were observed in 4 cases. No sequelae were observed in aseptic meningitis   cases. Four HSV encephalitis cases were monitored by PCR detection in CSF.   Despite acyclovir therapy, PCR remained positive in CSF up to 20 days in 2   cases. This result suggest that the antiviral treatment for HSV   encephalitis should be monitored by PCR detection of HSV in CSF. Copyright   2000 Wiley-Liss, Inc.

 

1814.  Nigrovic LE.  Chiang VW.  Cost analysis of enteroviral polymerase chain reaction in infants with   fever and cerebrospinal fluid pleocytosis [see comments].  Archives of Pediatrics & Adolescent Medicine.  154(8):817-21, 2000 Aug. 

Abstract

  BACKGROUND: Infants with fever and cerebrospinal fluid (CSF) pleocytosis   are routinely admitted to the hospital for parenteral antibiotic therapy   for potential bacterial meningitis pending results of CSF culture.   Published estimates suggest that 90% of all episodes of meningitis are   caused by enterovirus. Enteroviral polymerase chain reaction (ePCR) has a   sensitivity of 92% to 100% and a specificity of 97% to 100% in CSF.   

OBJECTIVE: To compare a management strategy using ePCR with current   practice to determine potential savings by allowing earlier discharge.   METHODS: Decision analysis comparing 2 strategies for the care of a   retrospective cohort of infants with fever and CSF pleocytosis: standard   practice vs ePCR testing of all CSF samples. Model assumptions include the   following: (1) standard practice patients continue parenteral antibiotic   therapy until CSF cultures are negative at 48 hours, (2) patients with  positive ePCR results would be discharged after 24 hours, (3) patients   with positive ePCR results have a negative CSF culture, and (4) costs are   calculated from actual patient charges with a cost-to-charge ratio of   0.65. SUBJECTS: All infants aged 28 days to 12 months admitted to an urban   teaching hospital with fever, CSF pleocytosis, and a negative CSF Gram   stain from January 1996 through December 1997. OUTCOME MEASURE: Total cost   of hospitalization. RESULTS: A total of 126 infants were identified. One   hundred twelve (89%) were discharged with a diagnosis of aseptic   meningitis; 72% of these cases occurred during the peak enterovirus season   (June to  October). Three of 3 patients with positive CSF cultures had   bacterial growth within 24 hours of admission. Mean length of stay for   patients with aseptic meningitis was 2.3 days (SD, +/-1.4 days). Total   cost of hospital care for all 126 infants was $381,145. In our patient   population, total patient costs would be reduced by the ePCR strategy if   enterovirus accounts for more than 5. 9% of all meningitis cases. Varying   the sensitivity of the ePCR assay from 100% to 90% changes the   "break-even" prevalence from 5.8% to 6.5%. Total cost savings of 10%, 20%,   and 30% would occur at an enteroviral meningitis prevalence of 36.3%,   66.7%, and 97.1%, respectively. CONCLUSIONS: Enteroviral PCR analysis of   CSF for infants admitted to the hospital with meningitis can result in   cost savings when the prevalence of enteroviral meningitis exceeds 5.9%.   Limiting use of ePCR to the enterovirus season would increase cost   savings. A prospective study is needed to validate these results. Arch   Pediatr Adolesc Med. 2000;154:817-821

 

1815.  Portegies P.  Corssmit N. Epstein-Barr virus and the nervous system.  Current Opinion in Neurology.  13(3):301-4, 2000 Jun. 

Abstract

  The neurological complications of Epstein-Barr virus infection include   viral meningitis, encephalitis and neuromuscular complications. The   introduction of cerebrospinal fluid polymerase chain reaction for   Epstein-Barr virus DNA has improved diagnosis of these conditions and of   primary central nervous system lymphoma in acquired immune deficiency   syndrome, and has enabled cerebrospinal fluid monitoring of therapy.   Prognosis remains good for most Epstein-Barr virus-related neurological  complications; for primary central nervous system lymphoma in acquired   immune deficiency syndrome the prognosis is still poor.

 

1816. Rajnik M.  Ottolini MG.  Serious infections of the central nervous system: encephalitis,   meningitis, and brain abscess.  Adolescent Medicine.  11(2):401-25, 2000 Jun.

Abstract

  Central nervous system infections in adolescents range from the diffuse   cerebritis of encephalitis to the regional inflammation of meningitis, and   very focal disease of brain abscess. Clinical presentations reflect this   wide spectrum, with encephalitis primarily characterized by altered mental   status, meningitis by fever, headache, and neck stiffness, and brain   abscess manifesting localizing findings. Encephalitis and viral meningitis   are frequently caused by the seasonal enteroviruses and arboviruses, while   most adolescent bacterial meningitis is due to Neisseria meningitidis and  Streptococcus pneumoniae. The microbiology of brain abscess reflects   underlying host risk factors. Gram-positive cocci are seen in patients   with congenital heart disease, while respiratory flora including anaerobes   are associated with sinus or otic disease. Lumbar puncture to characterize   and culture the CSF remains the optimal test for the diagnosis and   management of encephalitis and meningitis, while CT-guided needle biopsy   may be both diagnostic and therapeutic for brain abscesses. New diagnostic   tests include the use of PCR. A variety of safe and effective treatment   regimens exists for most bacterial infections as well as for some   herpesvirus infections. New vaccines are under study to further control   bacterial meningitis.   

 

1817.  Sanchez-Portocarrero J.  Perez-Cecilia E.  Corral O.  Romero-Vivas J.    Picazo JJ. The central nervous system and infection by Candida species.  Diagnostic Microbiology & Infectious Disease.  37(3):169-79, 2000 Jul.

 Abstract

  In this paper we have reviewed the main clinico-pathologic disease groups   of neurocandidiasis: the microabscesses, the macroabscesses, and the   meningitis. Special attention has been paid to the predisposing conditions   for the appearance of neurocandidiasis, the neuroimaging techniques, and   the study of the cerebrospinal fluid, needed for diagnosis. We have also   discussed the differential diagnosis with other illnesses. Treatment   should be given with amphotericin-B and 5-fluorocytosine. The use of other   antifungal drugs for neurocandidiasis is also discussed.

 

1818. Scott TF.  Brillman J.  Shunt-responsive dementia in sarcoid meningitis: role of magnetic   resonance imaging and cisternography.  Journal of Neuroimaging.  10(3):185-6, 2000 Jul.

Abstract

  The authors report a patient with progressive cognitive and gait decline   in association with sarcoid meningitis. The patient had evidence of active   inflammation as determined by cerebrospinal fluid examination and was   steroid dependent. Magnetic resonance imaging and radionucleotide   cisternography were complementary in establishing the diagnosis of   communicating hydrocephalus, and suggested that the patient would be shunt   responsive.  

 

1819.  Smith MB.  Boyars MC.  Veasey S.  Woods GL.  Generalized tuberculosis in the acquired immune deficiency syndrome.  Archives of Pathology & Laboratory Medicine.  124(9):1267-74, 2000 Sep.

Abstract

  OBJECTIVE: Generalized, or hematogenously disseminated, tuberculosis (TB)   in patients with the acquired immune deficiency syndrome (AIDS) has been   associated with a high incidence of cases remaining undiagnosed until   postmortem. To better characterize generalized TB in the setting of AIDS,   this report describes the clinical, laboratory, radiologic, and pathologic   features of 20 fatal cases. DESIGN: The medical records, autopsy   protocols, and histologic material from patients with AIDS and concomitant   TB were reviewed. All patients were autopsied at a tertiary care medical   center during the years 1985-1997. RESULTS: In 50% of our 20 cases,  diagnosis was not made until postmortem. Signs and symptoms were few,   including the absence of fever (temperature > or = 38 degrees C) in 55% of   patients. Consistent laboratory abnormalities of a nonspecific nature were   limited to hyponatremia (sodium <135 mmol/L) in 60%. Both peripheral and   deep (thoracic and abdominal) lymphadenopathy, unusual in adults with TB,   occurred in 45% and 95% of cases, respectively. In contrast to previous   reports, all of the 6 cases of tuberculous meningitis presented as acute   meningitis with a predominance of neutrophils in cerebrospinal fluid.   Necrotizing encephalitis with extension of the acute inflammation into the   superficial cortex was seen in all cases and tuberculous brain abscesses   occurred in 50% of cases, a higher frequency than previously reported.   Despite lung involvement in 90% of the cases, 33% of chest radiographs   were interpreted as normal and disseminated mycobacterial disease was not   suggested in the radiograph report in any of the other cases. Soft tissue   abscesses in uncharacteristic locations such as the neck, mediastinum, and   perirectal area occurred in these patients. Histologically, 95% of organs   sampled showed inflammatory foci characterized by extensive necrosis with   numerous neutrophils and/or karyorrhectic debris, numerous acid-fast   bacilli, few or no epithelioid histiocytes, and no Langhans giant cells.   CONCLUSION: Clinically and pathologically, generalized TB in the setting   of AIDS is characterized by either unusual features or a lack of the   typical features described for generalized TB in patients who do not have   AIDS. This absence of classic features contributes to the high incidence   of cases that remain undiagnosed until postmortem examination.  

 

1820. Tambussi G.  Gori A.  Capiluppi B.  Balotta C.  Papagno L.  Morandini B.    Di Pietro M.  Ciuffreda D.  Saracco A.  Lazzarin A.  Neurological symptoms during primary human immunodeficiency virus (HIV)   infection correlate with high levels of HIV RNA in cerebrospinal fluid. Clinical Infectious Diseases.  30(6):962-5, 2000 Jun. 

Abstract

  This analysis involves 22 patients with diagnosed symptomatic human   immunodeficiency virus (HIV) infection. Neurologic symptoms were present   in 11 patients, ranging from severe and persistent headache to clinical   signs suggestive of meningitis. A strong correlation between neurological   symptoms and cerebrospinal fluid (CSF) viral load was found. The mean CSF   HIV ribonucleic acid (RNA) level was 4. 12 log for patients with  neurological symptoms and 2.58 log for patients without neurological   symptoms (P<.00001). Plasma viral load alone does not correlate or predict   central nervous system (CNS) involvement. In our sample of patients, HIV   RNA levels could be detected in most patients regardless of the presence   of neurological symptoms. Moreover, early treatment including drugs with   high levels of penetration in the CNS must be considered for patients with   primary HIV infection.  

 

1821.  Tzeng YL.  Stephens DS.   Epidemiology and pathogenesis of Neisseria meningitidis. Microbes & Infection.  2(6):687-700, 2000 May.

Abstract

  Neisseria meningitidis, an exclusive pathogen of humans, remains the   leading worldwide cause of meningitis and fatal sepsis, usually in   otherwise healthy individuals. In recent years, significant advances have   improved our understanding of the epidemiology and genetic basis of   meningococcal disease and led to progress in the development of the next   generation of meningococcal vaccines. This review summarizes current   knowledge of the human susceptibility to and the epidemiology and   molecular pathogenesis of meningococcal disease.

 

1822. Yeat SW.  Mukari SZ.  Said H.  Motilal R.   Post meningitic sensori-neural hearing loss in children--alterations in   hearing level.  Medical Journal of Malaysia.  52(3):285-90, 1997 Sep.

Abstract

  Post meningitic sensori-neural hearing loss was studied in forty new cases   of bacterial meningitis and ten cases of viral meningitis treated at the   Pediatric  Institute, Kuala Lumpur Hospital from April 1991 to March 1992.   Hearing assessment at 2 weeks, 3 months and 6 months following the   diagnosis of meningitis using Brain Stem Evoked Response Audiometry showed   that hearing loss was prevalent only in patients with bacterial   meningitis. Hearing loss was detected in 32.5% of these patients during   the acute phase of the disease, 22.8% after 3 months and 24.2% after 6   months. In 63.6% of the affected cases, hearing loss was bilateral. In   61.5% of the patients who had hearing loss during the acute phase of the   disease, it was permanent, 16.7% had either partial or complete recovery   and, 15.4% had deterioration in hearing level. In 2 cases the subsequent   hearing level was unknown. The risk of developing sensori neural hearing   loss was found to be significantly higher in patients who developed other   neurological sequelae. The study highlights the importance of performing   repeated hearing assessment in children with bacterial meningitis and the   difficulty in appropriate selection of hearing aids in the early stages.

 

1823. Yoshida M.  Igarashi A.  Suwendra P.  Inada K.  Maha MS.  Kari K.  Suda H.    Antonio MT.  Arhana BNP.  Takikawa Y.  Maesawa S.  Yoshida H.  Chiba M.  The first report on human cases serologically diagnosed as Japanese   encephalitis in Indonesia.   Southeast Asian Journal of Tropical Medicine & Public Health.    30(4):698-706, 1999 Dec.

Abstract

  Although Japanese encephalitis (JE) virus was isolated from mosquitos in   1974, human JE cases have never been reported in Indonesia in spite of the   prevalence of anti-JE antibodies among human and pig populations as well   as abundant JE vector mosquitos. In this report, we describe serological   diagnosis of JE cases in Bali. Indonesia. using IgM-capture ELISA both on   serum and cerebrospinal fluid (CSF) of the patients. In the  first series  of our investigation (Series 1), we examined serum specimens from 12  patients with clinical diagnosis of viral encephalitis, meningitis or   dengue hemorrhagic fever (DHF), and found 2 possible JE cases. In the next   series (Series 2), we examined both serum and CSF from encephalitis   patients and gave laboratory diagnosis of JE. One of them was suspected to   have concomitant or recent infection with dengue virus, probably type 3.   These results strongly indicated that JE has been prevalent in Bali,   Indonesia.

 

2320. Anonymous. Meningococcal disease, African meningitis belt. Weekly Epidemiological Record.  76(8):57, 2001 Feb 23.

 

2321. Aponso D.  Bullen C.Presenting features of meningococcal disease, public health messages and media publicity: are they consistent?. New Zealand Medical Journal.  114(1127):83-5, 2001 Mar 9. 

Abstract

  AIMS: To investigate whether the presenting features of meningococcal  disease as promoted in public health awareness material and in the print  media accurately reflect the clinical features in patients admitted to  Auckland hospitals with meningococcal disease January 1998 to June 1999.  METHODS: Hospital record, public health message and newspaper article  review, with analysis by presenting feature, age group and disease  complex. RESULTS: The most common presenting features were fever (95%),  rash (65%), vomiting and nausea (64%), lethargy (62%), headache (44%),  refusing food and drink (35%), irritability (33%), muscle ache and joint  pains (27%) and stiff neck (26%). Public health messages gave appropriate  emphasis to the key features, whereas newspaper articles under-emphasised  these. The term 'meningitis' was used more frequently in newspapers (65%)  than in public health messages (30%), despite meningitis alone presenting  less frequently (38% of cases) than meningococcal septicaemia, and having  a less serious prognosis. CONCLUSIONS: Presenting features currently noted  in the Ministry of Health's health education resource material are  appropriate. Public health specialists dealing with the media should  ensure that appropriate messages are incorporated into media reports. A  greater use of the term 'meningococcal disease' by both public health  agencies and media would convey to the public the message that this  disease has a spectrum of presenting features, with those of septicaemia  more common, but also indicating an even greater need for urgency of  action than with 'classical' meningitic features.

 

2322. Aronin SI.  Quagliarello VJ. New perspectives on pneumococcal meningitis. [Review] [11 refs] Hospital Practice (Office Edition).  36(2):43-6, 49-50, 51, 2001 Feb 15.

 

2323. Awasthi M.  Patankar T.  Shah P.  Castillo M. Cerebral cryptococcosis: atypical appearances on CT. British Journal of Radiology.  74(877):83-5, 2001 Jan. 

Abstract

  Cryptococcal infection is common in immunocompromised patients, often  presenting with meningitis or meningoencephalitis. We report an unusual  presentation of cryptococcal infection in an immunocompetent patient  presenting with headache and hemiplegia. CT demonstrated a large  ring-enhancing lesion in the parietal region with intralesional  calcification.

 

2327. Chang DZ.  Correia NG.  Khurana P.  Tuthill RJ.  Taege AJ. A 35-year-old man with recurrent aseptic meningitis.  Cleveland Clinic Journal of Medicine.  68(3):199-201, 205-6, 2001 Mar. 

 

2328. Chin-Hong PV.  Sutton DA.  Roemer M.  Jacobson MA.  Aberg JA.Invasive fungal sinusitis and meningitis due to Arthrographis kalrae in a patient with AIDS. Journal of Clinical Microbiology.  39(2):804-7, 2001 Feb. 

Abstract

  We report the first described case of Arthrographis kalrae pansinusitis and meningitis in a patient with AIDS. The patient was initially diagnosed  with Arthrographis kalrae pansinusitis by endoscopic biopsy and culture.  The patient was treated with itraconazole for approximately 5 months and  then died secondary to Pneumocytis carinii pneumonia. Postmortem  examination revealed invasive fungal sinusitis that involved the sphenoid  sinus and that extended through the cribiform plate into the inferior  surfaces of the bilateral frontal lobes. There was also an associated  fungal meningitis and vasculitis with fungal thrombosis and multiple  recent infarcts that involved the frontal lobes, right caudate nucleus,  and putamen. Post mortem cultures were positive for A. kalrae. 

 

2329. Cullington HE. Light eye colour linked to deafness after meningitis. BMJ.  322(7286):587, 2001 Mar 10.

 

2331. Fobelo MJ.  Corzo Delgado JE.  Romero Alonso A.  Gomez-Bellver MJ. Aseptic meningitis related to valacyclovir. Annals of Pharmacotherapy.  35(1):128-9, 2001 Jan.

 

2332. Forgacs P.  Geyer CA.  Freidberg SR. Characterization of chemical meningitis after neurological surgery. Clinical Infectious Diseases.  32(2):179-85, 2001 Jan 15.

Abstract

  We reviewed the records of 70 consecutive adult patients with meningitis after a neurosurgical procedure, to determine the characteristics that  might help to distinguish a sterile postoperative chemical meningitis from  bacterial infection. The spinal fluid profiles in bacterial and chemical  meningitis are similar. The exceptions are that a spinal fluid white blood  cell count > 7500/microL (7500 x 10(6)/L) and a glucose level of < 10  mg/dL were not found in any case of chemical meningitis. The clinical  setting and clinical manifestations were distinct enough that no  antibiotic was administered after lumbar puncture to 30 (43%) of the 70  patients with postoperative meningitis. Chemical meningitis was infrequent  after surgery involving the spine and sinuses. Patients with chemical  meningitis did not have purulent wound drainage or significant wound  erythema or tenderness, coma, new focal neurological findings, or onset of  a new seizure disorder. They rarely had temperatures > 39.4 degrees C or cerebrospinal fluid rhinorrhea or otorrhea. 

 

2334. Gnanalingham MG.  Clarke MA. Primary Staphylococcus aureus meningitis in an infant. Journal of the Royal Society of Medicine.  94(2):86-8, 2001 Feb.

 

2336. Grossman SA.  Celano P. Images in clinical medicine. Neoplastic meningitis. New England Journal of Medicine.  344(7):494, 2001 Feb 15.

 

2337. Haase KK.  Lapointe M.  Haines SJ. Aseptic meningitis after intraventricular administration of gentamicin.  Pharmacotherapy.  21(1):103-7, 2001 Jan.

 

2338. Holt DE.  Halket S.  de Louvois J.  Harvey D. Neonatal meningitis in England and Wales: 10 years on. Archives of Disease in Childhood Fetal & Neonatal Edition.  84(2):F85-9,

  2001 Mar.

Abstract

  OBJECTIVES: To determine the incidence of neonatal meningitis in England  and Wales. DESIGN: A national postal survey using the British Paediatric  Surveillance Unit (BPSU) card scheme supplemented by information from  other sources. SETTING: England and Wales 1996-1997. SUBJECTS: A total of  274 babies less than 28 days of age who were treated for meningitis.  RESULTS: The incidence of neonatal meningitis in England and Wales has not  changed since our previous study in 1985-1987. However, the acute phase  mortality has fallen from 19.8% in 1985-1987 to 6.6% in this study. Group  B streptococci (42%) and Escherichia coli (16%) remain the most common  infecting microorganisms. Eight of 69 (12%) babies with group B  streptococci and 4/26 (15%) with E coli died. Antibiotic regimens based on  the third generation cephalosporins, notably cefotaxime, were most  commonly used (84%). The BPSU scheme identified 72% of cases during the  study period. Most cases of viral meningitis were not reported through the  BPSU. Less than a third of samples from aseptic meningitis were examined  for viruses; 56% of these were positive. CONCLUSIONS: Although the  incidence of neonatal meningitis remains unchanged, mortality from this  infection has fallen significantly. If this improvement is maintained as  reflected in the level of sequelae at 5 years of age, then the fear  surrounding meningitis during the neonatal period will have been dramatically reduced.   

 

2339. Hoque SN.  Graham J.  Kaufmann ME.  Tabaqchali S. Chryseobacterium (Flavobacterium) meningosepticum outbreak associated with colonization of water taps in a neonatal intensive care unit. Journal of Hospital Infection.  47(3):188-92, 2001 Mar. Abstract

  From September 1994 to May 1996, a strain of multi-resistant  Chryseobacterium (Flavobacterium) meningosepticum was isolated from eight neonates on a neonatal intensive care unit. The strain was resistant to ampicillin, ceftazidime, imipenem, gentamicin, ciprofloxacin and trimethoprim-sulphamethoxazole, susceptible to piperacillin and amikacin, and had variable susceptibility to rifampicin and vancomycin. Two neonates were infected (one had pneumonia and one septicaemia and meningitis); the remaining six neonates were colonized in the respiratory secretions. Two cases occurred that could not be explained by cross-infection during the outbreak. Environmental screening recovered C. meningosepticum from sink taps. Pulsed-field gel electrophoresis of chromosomal macrorestriction  digests of patient and environmental isolates showed them to be  representatives of a single strain. The outbreak was controlled after  staff were required to use an alcoholic handrub after washing hands, and toiletting of babies was done with sterile water instead of tap-water. Repair and chlorination of the water-tanks and changing the sink-taps resolves the outbreak. Copyright 2001 The Hospital Infection Society.

 

2340. Johnson JR.  Delavari P.  O'Bryan TT. Escherichia coli O18:K1:H7 isolates from patients with acute cystitis and neonatal meningitis exhibit common phylogenetic origins and virulence factor profiles. Journal of Infectious Diseases.  183(3):425-434, 2001 Feb 1. 

Abstract

  Escherichia coli isolates of serotype O18:K1:H7, taken from women with  acute cystitis, healthy control patients, and infants with neonatal  bacterial meningitis (NBM), were analyzed and were compared with  phylogenetically diverse control strains from the E. coli Reference  collection. Clonal relationships were defined by amplification  phylotyping, nicotinamide auxotrophy, and outer membrane protein patterns  (OMPs). Virulence factor profiles were determined by multiplex polymerase  chain reaction, probe hybridization, and hemagglutination testing. The  O18:K1:H7 cystitis, fecal, and NBM isolates were clonally derived. The  cystitis isolates and archetypal NBM isolates RS218 and C5 were from the  OMP6 subclone of E. coli O18:K1:H7 and exhibited a consensus virulence genotype that included papG allele III (cystitis-associated P fimbrial  adhesin), sfaS (S fimbrial adhesin), hlyA (hemolysin), cnf1 (cytotoxic  necrotizing factor), iroN (putative siderophore), and ibeA (invasion of  brain endothelium). The demonstrated commonality between O18:K1:H7  isolates from cystitis and NBM suggests common pathogenetic mechanisms and  the possibility of new approaches to prevention.

 

2341. Kawashima M.  Suzuki SO.  Yamashima T.  Fukui M.  Iwaki T. Prostaglandin D synthase (beta-trace) in meningeal hemangiopericytoma. Modern Pathology.  14(3):197-201, 2001 Mar. 

Abstract

  The level of prostaglandin D synthase (PGDS), a major protein constituent  of cerebrospinal fluid (CSF), is altered in various brain diseases,  including meningitis. However, its role in the brain remains unclear. PGDS  is mainly synthesized in the arachnoid cells, the choroid plexus and  oligodendrocytes in the central nervous system. Among brain tumors,  meningiomas showed intense immunoreactivity to PGDS in the perinuclear  region. Thus, PGDS has been considered a specific cell marker of  meningioma. In this study, we examined 25 meningeal hemangiopericytomas  (HPCs) and found that 16 of the tumors (64%) showed immunoreactivity for  PGDS in the perinuclear region. For comparison, 15 meningiomas, 14  soft-tissue HPCs, 1 mesenchymal chondrosarcoma, 3 choroid plexus  papillomas, and 7 oligodendrogliomas were also examined. Meningiomas  showed positive immunoreactivity for PGDS in 13 cases (80%). Except for one case located at the sacrum, none of the other soft-tissue HPCs showed immunostaining for PGDS. Mesenchymal chondrosarcoma arises in the bones of the skull, and its histological pattern resembles that of HPC; however, it showed no immunoreactivity for PGDS. Neither choroid plexus papillomas nor oligodendrogliomas were immunopositive for PGDS. These findings suggest that meningeal HPCs may have a unique molecular phenotype that is distinct from that of the soft-tissue HPCs. The origin of meningeal HPCs may be more closely related to the arachnoid cells.

 

2342. Kurup A.  Tee WS.  Loo LH.  Lin R. Infection of central nervous system by motile Enterococcus: first case report. Journal of Clinical Microbiology.  39(2):820-2, 2001 Feb. 

Abstract

  A 66-year-old man with four indwelling ventriculoperitoneal shunts for  multiloculated hydrocephalus from a complicated case of meningitis a year  before developed shunt infection based on a syndrome of fever, drowsiness,  and cerebrospinal fluid neutrophil pleocytosis in the background of  repeated surgical manipulation to relieve successive shunt blockages. The  cerebrospinal fluid culture, which yielded a motile Enterococcus species,  was believed to originate from the gut. This isolate was lost in storage  and could not be characterized further. The patient improved with  vancomycin and high-dose ampicillin therapy. He relapsed a month later  with Enterococcus gallinarum shunt infection, which responded to high-dose  ampicillin and gentamicin therapy. This is probably the first case report  of motile Enterococcus infection of the central nervous system. 

 

2343. Lai KK. Enterobacter sakazakii infections among neonates, infants, children, and adults. Case reports and a review of the literature. [Review] [41 refs] Medicine.  80(2):113-22, 2001 Mar. 

Abstract

  Enterobacter sakazakii can cause serious infections especially among the  very young and the elderly. It continues to be more common among neonates and infants than adults. Its tropism for the central nervous system in neonates and infants remains a mystery. Among neonates and infants, E. sakazakii has a propensity to cause meningitis resulting in ventriculitis, brain abscess or cyst formation, and development of hydrocephalus requiring ventricular-peritoneal shunt. Computed tomography of the head is therefore useful in following patients with E. sakazakii meningitis. Mortality and morbidity of E.  sakazakii meningitis is high, and virtually all patients recovering from the central nervous system infection suffered mental and physical developmental delays. The case-fatality rate decreased among patients with meningitis treated with the third-generation  cephalosporins. Most adults with E. sakazakii infection had serious underlying diseases and 50% of the adults with the infection had malignancies. However there has never been a known case of meningitis. Increasing antibiotic resistance among Enterobacter species should lead one to consider using the carbapenems or the newer cephalosporins in combination with a second agent such as an aminoglycoside. Limited data suggest that trimethoprim-sulfamethoxazole may be a useful agent in the treatment of infections caused by the Enterobacter species, especially in view of the production of extended-spectrum beta-lactamases capable of inactivating the cephalosporins and extended-spectrum penicillin. 

 

2344. Liebeskind DS.  Ostrzega N.  Wasterlain CG.  Buttner EA. Neurologic manifestations of disseminated infection with Mycobacterium abscessus.  Neurology.  56(6):810-3, 2001 Mar 27.

Abstract

  Mycobacterium abscessus is a ubiquitous, saprophytic organism with low pathogenic potential. The authors describe the previously unreported  clinical features of meningitis and native valve endocarditis caused by  this rapidly growing atypical mycobacterium. The fatal outcome of this  unusual case coincides with the grim prognosis of this disseminated  infection and the significant mortality rate associated with neurologic complications of infective endocarditis. 

2345. Lortholary O.  Dromer F.  Mathoulin-Pelissier S.  Fitting C.  Improvisi L.   Cavaillon JM.  Dupont B.  French Cryptococcosis Study Group. Immune mediators in cerebrospinal fluid during cryptococcosis are influenced by meningeal involvement and human immunodeficiency virus serostatus. Journal of Infectious Diseases.  183(2):294-302, 2001 Jan 15.

Abstract

  Pro- and anti-inflammatory mediators (tumor necrosis factor [TNF]-alpha,  interleukin [IL]-6, IL-8, IL-10, and soluble TNF receptor II [sTNFR] II) were measured in cerebrospinal fluid (CSF) before treatment (day 0), and after 2 weeks and 3 months of antifungal therapy in 51 human immunodeficiency virus (HIV)-positive and 7 HIV-negative patients with culture-confirmed cryptococcosis. On day 0, all mediator concentrations, except IL-10 in HIV-positive patients, were higher in patients with meningeal, rather than extrameningeal cryptococcosis or in control subjects (P<.05). For meningitis patients, all mediator levels, except sTNFR II, were higher in HIV-negative than HIV-positive patients (P<.05). Day 0 CSF IL-8 levels were higher in HIV-positive patients receiving antiretroviral therapy than in untreated persons (P<.02). Day 0 sTNFR II levels were higher in HIV-positive survivors at 3 months, and elevated levels were sustained in HIV-positive patients with meningitis. Overall, these data support the idea that inflammatory responses are crucial to the eradication of cryptococcal infections in the central nervous system. 

 

 

2347. Manfredi R.  Nanetti A.  Valentini R.  Chiodo F. Acinetobacter infections in patients with human immunodeficiency virus infection: microbiological and clinical epidemiology. Chemotherapy.  47(1):19-28, 2001 Jan-Feb. 

Abstract

  BACKGROUND: We evaluated the role of complications caused by Acinetobacter spp. in the setting of HIV infection. METHODS: Clinical records of 1,923  consecutive HIV-infected patients hospitalized in a 9-year period were  retrospectively reviewed, in order to identify all cases of Acinetobacter  spp. complications, and to assess their occurrence and outcome according  to several epidemiological, clinical and laboratory parameters.  RESULTS:  Ten patients out of 1,923 (0.52%) developed Acinetobacter spp. infections:  sepsis in four cases, urinary tract infection in three, pneumonia in two  and septicaemic pneumonia in the remaining patient. All patients were  severely immunocompromised, as shown by a mean CD4+ lymphocyte count of  122 cells/microl and a frequent prior diagnosis of AIDS. As opposed to  other infections, septicaemia was associated with a significantly lower  CD4+ cell count and a more frequent occurrence of neutropenia.  Hospital-acquired Acinetobacter spp. infections were significantly more  frequent than community-acquired ones, and prevailingly involved patients  with AIDS and leucopenia, being responsible for frequent blood dissemination. Antimicrobial, corticosteroid and cotrimoxazole treatment were frequently carried out during the month preceding disease onset. Antibiotic susceptibility studies proved the complete resistance of microbial isolates to ampicillin and cephalothin and poor sensitivity to second-generation cephalosporins and gentamicin, while greater susceptibility was shown to ceftazidime, netilmicin and amikacin, followed by piperacillin, cotrimoxazole and quinolones. Appropriate antimicrobial treatment led to clinical and microbiological cure in all cases, with no related mortality or relapses. CONCLUSIONS: Since only 23 episodes of HIV-associated Acinetobacter spp. infections have been described to date in 11 different reports (nine cases of bacteraemia, eight of pneumonia,  two of urinary tract involvement, one of intravenous access device infection, one of meningitis and two with unspecified localization), our series represents the largest one dealing with HIV-associated Acinetobacter spp. infections. According to our experience, Acinetobacter spp. may be responsible for appreciable morbidity among patients with HIV infection, above all when a low CD4+ cell count, neutropenia and hospitalization are present. Clinicians and microbiologists who work in the field of HIV infection should consider the potential pathogenic role of Acinetobacter spp. organisms even in the absence of some presumed risk factors, because of the relationship between these infections and immunodeficiency, hospitalization, other infectious complications, prior antibiotic and steroid treatment and extended antimicrobial resistance  patterns. Copyright 1999 S. Karger AG, Basel

 

2348.  McMinn P.  Stratov I.  Nagarajan L.  Davis S. Neurological manifestations of enterovirus 71 infection in children during an outbreak of hand, foot, and mouth disease in Western Australia. Clinical Infectious Diseases.  32(2):236-42, 2001 Jan 15. 

Abstract

  Enterovirus 71 (EV71) causes epidemics of hand, foot, and mouth disease associated with neurological complications in young children. We report an  outbreak of EV71-associated neurological disease that occurred from  February through September 1999 in Perth, Western Australia. Fourteen  children with culture-proven, EV71-induced neurological disease were  identified. Nine patients (64%) developed severe neurological disease; 4  of these patients developed long-term neurological sequelae. Neurological  syndromes included aseptic meningitis, Guillain-Barre syndrome, acute  transverse myelitis, acute cerebellar ataxia, opso-myoclonus syndrome,  benign intracranial hypertension, and a febrile convulsion. Clinical and  magnetic resonance imaging data indicated that immunopathology was a major  factor in the pathogenesis of neurological disease in this outbreak. This  finding is in contrast to reports of previous EV71 epidemics, in which  virus-induced damage to gray matter was the most frequent cause of  neurological disease. 

 

2349. Merkelbach S.  Konig J.  Rohn S.  Muller M. The use of clinical scales in depicting cerebrovascular complications in bacterial meningitis. Journal of Neuroimaging.  11(1):25-9, 2001 Jan.  

Abstract

  To evaluate the utility of different scales for clinical assessment, over time, in acute bacterial meningitis, the authors investigated 53 consecutive patients (mean age 53 +/- 17 years). Clinical status on days 1, 3, 5, 8, and 14 after admission was determined by the Scandinavian Stroke Scale (SSS), Glasgow Coma Scale (GCS), and Hunt and Hess Scale (HH), and, on day 21, by the Glasgow Outcome Scale (GOS). Transcranial Doppler examinations were performed serially to assess for disease-related arterial narrowing. This was observed in 27 patients (51%) within 2 weeks of admission. All scales were observed to correlate with the 21-day GOS. Patients with cerebral arterial narrowing had significantly decreased SSS scores between days 1 and 8 and worse GCS and HH scores between days 3 and 5. Mean blood flow velocity in the middle cerebral artery on days 5 and 8 significantly correlated with GOS (r = 0.268, P < .008; r = -0.324, P < .003, respectively). The use of such clinical scales allows standardized assessment of patients with bacterial meningitis and provides prognostic information. Cerebral arterial narrowing was observed to correlate with neurologic impairment.

 

2350. Muller MP.  Richardson DC.  Walmsley SL. Trimethoprim-sulfamethoxazole induced aseptic meningitis in a renal transplant patient. [Review] [20 refs] Clinical Nephrology.  55(1):80-4, 2001 Jan. 

Abstract

  A 45-year-old man underwent renal transplant for end-stage renal disease  complicating systemic lupus erythematosis. Within 24 hours of initiating  Pneumocystis carinii pneumonia (PCP) prophylaxis with trimethoprim-sulfamethoxazole (TMP-SMX) he developed fever and confusion. Cerebrospinal fluid examination revealed a pleocytosis but cultures were negative. The patient improved within three days after cessation of the TMP-SMX but symptoms recurred rapidly upon drug rechallenge. Drug-induced aseptic meningitis is an uncommon but well described clinical entity. This is the first case described in a patient following renal transplantation. The literature is reviewed and the clinical features, diagnostic challenges and possible mechanisms of TMP-SMX-induced aseptic meningitis are discussed. This problem may be more common in the transplant population than is recognized given the difficulty of diagnosis combined with the widespread use of TMP-SMX as PCP prophylaxis. 

 

 

2352. Panciera RJ.  Ewing SA.  Confer AW. Ocular histopathology of Ehrlichial infections in the dog. Veterinary Pathology.  38(1):43-6, 2001 Jan. 

Abstract

  Histologic examination of eyes and brains of 27 dogs experimentally infected with either Ehrlichia canis, E. ewingii, E. chaffeensis, or human  granulocytic ehrlichia (HGE) was conducted in the course of several  experiments, the primary objectives of which were to investigate the  susceptibility of the domestic dog to infection with various ehrlichial  species and to assess the ability of ixodid tick species to acquire and  transmit those infections. Uveitis and meningitis occurred in each of the  dogs infected with E. canis but was not observed in dogs infected with the  other Ehrlichia species. The inflammatory infiltrate was predominantly  lymphocytic, monocytic, and plasmacytic; granulocytes were notably few.  Ocular inflammation was most common and most intense in the ciliary body,  becoming less intense in the choroid, iris, and retina, respectively.  Meningitis was often accompanied by mild neuroparenchymal vascular cuffing  and gliosis. The meningeal inflammatory cell infiltrate included a  prominent monocyte population. Ocular and meningeal lesions were present  in all E. canis-infected dogs from 22 through 200 days postexposure.  Neither ocular nor brain lesions were observed with any of the other ehrlichial infections.

 

2353. Ratka A.  Erramouspe J. Intramuscular ceftriaxone in the treatment of childhood meningitis due to Haemophilus influenzae type F. Annals of Pharmacotherapy.  35(1):36-40, 2001 Jan.

Abstract

  OBJECTIVE: To describe a case of meningitis caused by Haemophilus  influenzae type f (Hif) in a child. CASE SUMMARY: A 2.5-year-old white girl (18 kg) was hospitalized because of acute ataxia. The cerebrospinal fluid culture grew H. influenzae, which was later identified as type f. Therapy was limited by the inability to gain intravenous access. Treatment was initiated with dexamethasone 8 mg (0.44 mg/kg) intramuscularly, one dose on the day prior to initiation of ceftriaxone therapy, and intramuscular ceftriaxone 2 g (111 mg/kg/dose) once a day. After the first day, dexamethasone was administered at 3 mg (0.17 mg/kg/d) orally four times per day for four days. Within two days, the patient became afebrile and improved significantly. The remaining treatments were given during  daily hospital visits on an outpatient basis. No complications occurred during the follow-up visits. DISCUSSION: The clinical presentation and therapeutic management of Hif meningitis is similar to that of H. influenzae type b (Hib) meningitis. Factors that may predispose a child to infections caused by Hif include upper respiratory tract infections, day care attendance, Down syndrome, and immunodeficiency. Hif meningitis  usually is treated with a third-generation cephalosporin (frequently  ceftriaxone). Although most often administered intravenously, intramuscular ceftriaxone can provide a satisfactory clinical outcome in a child with adequate peripheral perfusion but limited intravenous access.  The majority of reported cases of Hif meningitis resolve with appropriate antibiotic therapy; however, long-term neurologic sequelae occasionally  occur. CONCLUSIONS: Hif occasionally causes pediatric meningitis. In a  patient with good perfusion and difficult intravenous access, daily  intramuscular administration of ceftriaxone can be an effective treatment  option. In this case, Hif meningitis occurred abruptly and resolved within  48 hours of initiation of ceftriaxone and dexamethasone without long-term  sequelae. The risks of giving dexamethasone appear to be minimal, although  efficacy for preventing Hif complications remains to be proven.

 

2354. Ravenscroft A.  Schoeman JF.  Donald PR. Tuberculous granulomas in childhood tuberculous meningitis: radiological features and course. Journal of Tropical Pediatrics.  47(1):5-12, 2001 Feb.

Abstract

  The clinical course and serial cranial computerized tomographic (CT) findings of 202 children with tuberculous meningitis (TBM) admitted to Tygerberg Hospital between 1985 and 1994 were reviewed with regard to the incidence, CT appearance and clinical course of associated intracranial  tuberculous granulomas. Thirty-four patients (16.85 per cent) had  associated intracranial granulomas. Thirty-eight individual lesions were  analysed and classified as meningeal, parenchymal or ependymal according  to their central nervous system (CNS) location. Twenty-five patients had  round to irregular, brain iso-, hypo- or hyperdense meningeal granulomas with variable degrees of enhancement and peri-lesional hypodensities. Four  patients had diffusely enhancing, brain isodense, enplaque-like ependymal  granulomas associated with the ventricular ependymal lining. Four patients with miliary tuberculosis and TBM showed multiple small diffusely enhancing, brain iso- or hyperdense parenchymal lesions and associated hypodensities on initial CT. Although granulomas in the meningeal and ependymal group had the propensity to paradoxically enlarge or appear on standard four-drug antituberculosis therapy, the majority resolved uneventfully. Rapid resolution of small parenchymal granulomas associated with miliary tuberculosis occurred in all cases. Most granulomas in this series were co-incidental, asymptomatic CT findings. In rare cases, the development or enlargement of a strategically located granuloma may result in complications.

 

2355. Rees JH.  Balakas N.  Agathonikou A.  Hain SF.  Giovanonni G. Panayiotopoulos CP.  Luxsuwong M.  Revesz T. Primary diffuse leptomeningeal gliomatosis simulating tuberculous meningitis.Journal of Neurology, Neurosurgery & Psychiatry.  70(1):120-2, 2001 Jan.

Abstract

  Three patients are reported on who presented with communicating hydrocephalus due to presumed tuberculous meningitis. Subsequent clinical deterioration despite antituberculous chemotherapy prompted reassessment with FDG-PET scanning and meningeal biopsy in one case and repeat CSF cytology with special staining in the second. The third patient died and postmortem confirmed a diagnosis of primary diffuse leptomeningeal gliomatosis. In the first two patients, MRI of the entire neuraxis showed no evidence of a primary intraparenchymal tumour. These cases emphasise the need for repeated reassessment in patients with culture negative lymphocytic meningitis. In addition, this is the first report of FDG-PET scanning in leptomeningeal gliomatosis.

 

2356. Sacktor N.  Lyles RH.  Skolasky R.  Kleeberger C.  Selnes OA.  Miller EN.  Becker JT.  Cohen B.  McArthur JC.  The Multicenter AIDS Cohort Study. HIV-associated neurologic disease incidence changes:: Multicenter AIDS Cohort Study, 1990-1998. Neurology.  56(2):257-60, 2001 Jan 23.

Abstract

  This study examined the temporal trends in the incidence rates of HIV dementia, cryptococcal meningitis, toxoplasmosis, progressive multifocal  leukoencephalopathy, and CNS lymphoma from January 1990 to December 1998  in the Multicenter AIDS Cohort Study. The incidence rates for HIV  dementia, cryptococcal meningitis, and lymphoma decreased following the  introduction of highly active antiretroviral therapy (HAART). The  proportion of new cases of HIV dementia with a CD4 count in a higher range  (i.e., 201 to 350) since 1996 may be increasing.

 

2357. Sakhuja V.  Sud K.  Kalra OP.  D'Cruz S.  Kohli HS.  Jha V.  Gupta K. Vasishta RK. Central nervous system complications in renal transplant recipients in a  tropical environment. Journal of the Neurological Sciences.  183(1):89-93, 2001 Jan 15.

Abstract

  Renal transplant recipients are at risk of developing various infectious  and non-infectious complications affecting the central nervous system (CNS). There is paucity of data regarding the spectrum of CNS complications and the epidemiology of infective agents varies according to geographical location. We retrospectively studied the spectrum of CNS complications seen in 792 renal allograft recipients followed up at this tertiary care centre in north India over a 19-year period. Autopsy findings of 78 allograft recipients who died in the hospital were also reviewed and included. The brain was examined in 22 of these patients. Overall, 79 (10%) patients developed some form of CNS dysfunction with a  mortality rate of 60.8%. CNS infections occurred in 31 renal allograft recipients (3.9% of total) and accounted for the largest group (39.2%). Fungi were the commonest etiological agents (21 patients) and were  associated with a 70% mortality, with cryptococcal meningitis occurring in  12, mucormycosis in six, aspergillosis in one, and other unusual fungal  infections in the remaining two patients. All patients with mucormycosis  had a fatal outcome. The second largest group comprised of patients with  non-uremic encephalopathies (23 patients, 29.1%) with metabolic  encephalopathy occurring in 13, toxic encephalopathy in nine and  hypertensive encephalopathy in one patient) and was associated with an  overall mortality rate of 60.9%. Cerebrovascular accidents occurred in 12  patients (15.2%) and were associated with a mortality of 91.7%. Other CNS  complications included treatment related complications in four (5.1%),  primary CNS lymphomas in three (3.8%), and miscellaneous complications in six patients (7.6%). Patients with non-cryptococcal fungal infections of the CNS, hepatic and toxic encephalopathy and those with cerebrovascular accidents had the worst outcome. There was no relationship between the development of infection or stroke and the type of maintenance immunosuppression used. We conclude that complications involving the CNS occur in 10% of all renal transplant recipients and are associated a with  high mortality, warranting early diagnosis and aggressive treatment.

 

2358. Socan M.  Ravnik I.  Bencina D.  Dovc P.  Zakotnik B.  Jazbec J. Neurological symptoms in patients whose cerebrospinal fluid is culture- and/or polymerase chain reaction-positive for Mycoplasma pneumoniae. Clinical Infectious Diseases.  32(2):E31-5, 2001 Jan 15.

Abstract

  We describe 13 patients with neurological signs and symptoms associated  with Mycoplasma pneumoniae infection. M. pneumoniae was isolated from the cerebrospinal fluid (CSF) of 9 patients: 5 with meningoencephalitis, 2  with meningitis, and 1 with cerebrovascular infarction. One patient had  headache and difficulties with concentration and thinking for 1 month after the acute infection. M. pneumoniae was detected, by means of PCR, in  the CSF of 4 patients with negative culture results. Two had epileptic seizures, 1 had blurred vision as a consequence of edema of the optic disk, and 1 had peripheral nerve neuropathy.

 

2359. Tinsley C.  Nassif X. Meningococcal pathogenesis: at the boundary between the pre- and post-genomic eras. [Review] [49 refs] Current Opinion in Microbiology.  4(1):47-52, 2001 Feb.

Abstract

  Meningococcal disease remains an important public health burden worldwide  and, indeed, cause of death, particularly in poorer countries. The rapidly  progressive nature of infections means that antibiotic therapy often comes  too late. Vaccines are of limited efficacy in infants, one of the most  vulnerable age groups, and do not exist for bacteria of serogroup B.  Hence, much remains to be achieved in terms of vaccine design and the  understanding of the pathogenesis of meningococcal disease. The causative  bacterium, Neisseria meningitidis, is usually a commensal of the  nasopharynx. Factors that lead to the invasion of the bloodstream, often  followed by the crossing of the blood-brain barrier and meningitis, may be  partly host- and partly bacterium-dependent, but are ill-understood. It is  hoped that, taken together with the fundamental knowledge gained from  biochemical and genetic studies, the huge amount of new information made  available with the recent publication of the genome sequences will help to  unlock more of the secrets of the lifestyle and pathogenic potential of  this still poorly understood pathogen. [References: 49]

 

2361. Wadia RS.  Pujari SN.  Kothari S.  Udhar M.  Kulkarni S.  Bhagat S. Nanivadekar A. Neurological manifestations of HIV disease. Journal of the Association of Physicians of India.  49:343-8, 2001 Mar.

Abstract

  We report the results of neurological evaluation of 1,527 HIV positive  subjects. Neurological complications were seen in 457 patients (481  neurological events). The prevalence was 20.24% of patients attending the  out-patient clinic and in 44.57% of in-patients. Involvement of all levels  of neuraxes was documented. The commonest manifestations were  neuropathies, including herpes zoster (28.27%), meningitis (17.88%) and  mass lesions (16%). Cryptococcal meningitis was clearly commoner than  tubercular meningitis (67.44% vs 18.60% of all cases of meningitis,  respectivelv). Amongst mass lesions, 14/24 single lesions and 27/38  multiple lesions responded to anti-toxoplasma treatment and were diagnosed  as CNS toxoplasmosis. In abscence of biopsy, it would be prudent to  initiate empirical anti-toxoplasma treatment for all HIV patients with  mass lesions and assess clinical and radiological response. To our  knowledge this is the largest series of neurological manifestations of HIV disease documented in Indian literature.

 

2362. Wang CC.  Siu LK.  Chen MK.  Yu YL.  Lin FM.  Ho M.  Chu ML. Use of automated riboprinter and pulsed-field gel electrophoresis for  epidemiological studies of invasive Haemophilus influenzae in Taiwan. Journal of Medical Microbiology.  50(3):277-83, 2001 Mar.

Abstract

  A total of 87 invasive isolates of Haemophilus influenzae isolated throughout Taiwan from 1994 to 1998 was collected; 57 were from children  <14 years old. In all, 60.9% of isolates were resistant to ampicillin and  produced beta-lactamase. Ribotyping revealed six different profiles in 55  isolates of type b, nine profiles in 10 isolates of non-type b and 12  profiles in 22 isolates of non-typable H. influenzae. Among isolates from  35 cases of meningitis, 30 (86%) were in ribogroups 1, 2 and 3 with >90%  genetic similarity. Compared with all the other ribogroups, ribogroups 1,  2 and 3, which encompassed all H. influenzae type b, were significantly  more prevalent as a cause of meningitis in children <14 years old. Further  subtyping of the predominant ribogroup by pulsed-field gel electrophoresis  (PFGE) identified differences of 0-6 bands among these isolates of  ribogroup 1, which indicated distant relatedness. Automated ribotyping was  found to be a useful method and was less time-consuming for molecular  epidemiology studies of H. influenzae. PFGE is suggested as an addition to  ribotyping to improve discrimination if H. influenzae type b is involved.  Differentiating ribogroups between type b and non-type b H. influenzae by  genotyping may help to understand the molecular characteristics of  outbreaks, endemicity and value of vaccination. According to the results  of ribotyping and PFGE, it seems possible that spread of invasive H.  influenzae type b had occurred and ribotyping confirmed that there was no clonal spread of non-type b H. influenzae in Taiwan.

 

2363. Wong M.  Schlaggar BL.  Landt M. Postictal cerebrospinal fluid abnormalities in children. Journal of Pediatrics.  138(3):373-7, 2001 Mar.

Abstract

  OBJECTIVES: To determine the frequency and characteristics of seizure-induced cerebrospinal fluid (CSF) abnormalities in children and to identify potential alternative causes of these findings. METHODS: Consecutive patients (n = 80) who underwent lumbar puncture within 24 hours after a seizure were studied retrospectively. The presence of CSF abnormalities in total leukocytes, polymorphonuclear cells, and protein  was determined by using age-specific reference values. Coexisting  conditions that could affect CSF findings, such as traumatic lumbar  puncture, concurrent neurologic disease, and undiagnosed meningitis, were  identified. RESULTS: Eighteen of the 80 patients were excluded from the  final study group because of the presence of another condition that could  alter the CSF. More than 50% of the excluded patients had an abnormal CSF  leukocyte count or protein level, including 2 patients with initially  undiagnosed meningitis, which was subsequently detected by post-hoc  polymerase chain reaction testing. In the remaining 62 patients, postictal  pleocytosis was detected in only 3 (5%), and increased protein was  detected in only 6 (10%). The maximal postictal pleocytosis and protein  level were 8 x 10(6) leukocytes/L (8 leukocytes/mm(3)) and 0.52 g/L (52  mg/dL), respectively. CONCLUSIONS: Seizure-induced CSF abnormalities are  rare in children, and alternative, often unidentified, disease processes  may account for many observed postictal abnormalities. All patients with  abnormal CSF after a seizure should be thoroughly evaluated for other  causes of the abnormality.

 

 

2858.    Cellerini M.  Gabbrielli S.  Maddali Bongi S.  Cammelli D. MRI of cerebral rheumatoid pachymeningitis: report of two cases with follow-up. Neuroradiology.  43(2):147-50, 2001 Feb.

Abstract

  We report the clinical and neuroradiological features of cerebral rheumatoid pachymeningitis with 1 year follow-up in two patients. MRI of the head enabled noninvasive diagnosis of both the meningeal abnormality and its complications, consisting of hypertensive hydrocephalus and superior sagittal sinus thrombosis, respectively. Dural sinus thrombosis, very uncommon in rheumatoid arthritis, was confirmed by phase-contrast MRA. Worsening of the pachymeningitis at follow-up was observed in both patients despite regression or stability of the clinical picture and long-term therapy.

 

2859.    Chaturvedi P.  Kishore M. Modified Glasgow Coma Scale to predict mortality in febrile unconscious children. Indian Journal of Pediatrics.  68(4):311-4, 2001 Apr.

Abstract

  A prospective hospital based study was conducted in the Department of Pediatrics of the Kasturba Hospital, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha to predict the mortality in children admitted with fever and unconsciousness using the Modified Glasgow Coma Scale (MGCS) score. Forty eight children were admitted with fever and unconsciousness; cases of febrile convulsions, epilepsy and cerebral palsy were excluded. MGCS scores were assessed on admission and repeated at 12 hours, 24 hours, 48 hours and 72 hours after admission in each case. Diagnosis in each case was confirmed by history, examinations and investigations. All the cases were regularly followed up till death/discharge. The overall mortality was 29.1% (14/48) out of which 85% (12/14) died within the first 24 hours. Mortality was highest in the toddler age group and in patients with pyogenic meningitis. There was a significant association between death and MGCS scores on admission with a post test probability for discharge being only 10% with a score of less than 5 and 99% with a score of more than 10 respectively. MGCS scores on admission can be used to predict mortality in patients hospitalized with fever and unconsciousness. The scale is simple, easy, can be applied at bed side and does not need any investigations. Its application in developing countries with limited investigative and intensive care facilities can help the treating physician decide regarding referral and counseling the parents regarding the probable clinical outcome.

 

2860.    Henquell C.  Chambon M.  Bailly JL.  Alcaraz S.  De Champs C.  Archimbaud C.  Labbe A.  Charbonne F.  Peigue-Lafeuille H. Prospective analysis of 61 cases of enteroviral meningitis: interest of systematic genome detection in cerebrospinal fluid irrespective of cytologic examination results.  Journal of Clinical Virology.  21(1):29-35, 2001 Apr.

Abstract

  BACKGROUND: Enteroviruses are the most commonly identified cause of viral meningitis. Detection of the enterovirus genome in cerebrospinal fluid (CSF) using reverse-transcription polymerase chain reaction (PCR) has proved to be useful in diagnosis and is more rapid and sensitive than viral cultures. In routine practice, cytologic examination results of CSF are obtained swiftly and PCR indication is performed as a second step. OBJECTIVES: The aim of this study was to determine, by analysis of complete data from CSF results for 61 cases of proven enteroviral meningitis, whether cytologic CSF findings can be used to establish viral etiology and to indicate if PCR assay should be performed. STUDY DESIGN: From a prospective study of children admitted during 1997 for suspected enterovirus meningitis in which PCR and viral cultures of CSF were systematically performed, we selected 61 patients with proven enterovirus meningitis. We compared global white cell count (WCC), relative percentage of lymphocytes/neutrophils, PCR and culture for enterovirus, patient age, and clinical data. RESULTS: 92% of patients (56/61) had positive PCR in CSF and in 48% (29/61) enterovirus was isolated in CSF. Nine patients (14.75%) had WCC<10/mm(3); eight of them had positive PCR and two had positive culture. There were comparable numbers of CSF with a predominance of lymphocytes (n=25) and CSF with a predominance of neutrophils (n=22), and of positive PCR and positive cultures of CSF in the two groups. Results were not influenced by the age of the patients. CONCLUSION: Irrespective of other CSF parameters, it seems difficult to dispense with PCR assay for enterovirus genome detection. It should be introduced as a true rapid routine test. Early reporting of a positive PCR result could result in a considerable saving in health resources.

 

2861.    Kashyap AS.  Kashyap S.  A 35 year man with acromegaly and neck stiffness. Postgraduate Medical Journal.  77(906):269-70; discussion 279-80, 2001 Apr.

 

2862.    Kolb-Maurer A.  Pilgrim S.  Kampgen E.  McLellan AD.  Brocker EB.  Goebel W.  Gentschev I. Antibodies against listerial protein 60 act as an opsonin for phagocytosis of Listeria monocytogenes by human dendritic cells. Infection & Immunity.  69(5):3100-9, 2001 May.

Abstract

  Human-monocyte-derived dendritic cells (MoDC) are very efficient in the uptake of Listeria monocytogenes, a gram-positive bacterium which is an important pathogen in humans and animals causing systemic infections with symptoms such as septicemia and meningitis. In this work, we analyzed the influence of blood plasma on the internalization of L. monocytogenes into human MoDC and compared the uptake of L. monocytogenes with that of Salmonella enterica serovar Typhimurium and Yersinia enterocolitica. While human plasma did not significantly influence the uptake of serovar Typhimurium and Y. enterocolitica by human MoDC, the efficiency of the uptake of L. monocytogenes by these phagocytes was strongly enhanced by human plasma. In plasma-free medium the internalization of L. monocytogenes was very low, whereas the addition of pooled human immunoglobulins resulted in the internalization of these bacteria to a degree comparable to the highly efficient uptake observed with human plasma. All human plasma tested contained antibodies against the 60-kDa extracellular protein of L. monocytogenes (p60), and anti-p60 antibodies were also found in the commercially available pooled immunoglobulins. Strikingly, in contrast to L. monocytogenes wild type, an iap deletion mutant (totally deficient in p60) showed only a minor difference in the uptake by human MoDC in the presence or the absence of human plasma. These results support the assumption that antibodies against the listerial p60 protein may play an important role in Fc-receptor-mediated uptake of L. monocytogenes by human MoDC via opsonization of the bacteria. This process may have a major impact in preventing systemic infection in L. monocytogenes in immunocompetent humans.

 

2863.    Lan SH.  Chang WN.  Lu CH.  Lui CC.  Chang HW. Cerebral infarction in chronic meningitis: a comparison of tuberculous meningitis and cryptococcal meningitis. QJM.  94(5):247-53, 2001 May.

Abstract

  Twenty-eight patients with cerebral infarction secondary to chronic meningitis were retrospectively identified at our institution over a period of 5 years. They accounted for 47% (17/36) of tuberculous meningitis (TBM) and 32% (11/34) of cryptococcal meningitis cases. Single infarctions were found in 15 patients and multiple infarctions in 13. The distribution of single infarctions was: basal ganglia 7; internal capsule 3; thalamus 1; cerebellum 1; and cortical infarct 3. Therapeutic outcomes at 3 months were determined using a modified Barthel INDEX: At follow-up of 3 months or more, 10 had good outcomes while the other 18 had poor outcomes. The 18 with poor outcomes included six who died, and 12 who had severe neurological sequelae. TBM and cryptococcal meningitis shared similar clinical features, both being frequently associated with other neurological complications, including hydrocephalus, cranial nerve palsy, and seizures in our patients. However, extracranial involvement, such as spinal and pulmonary involvement, was more commonly found in TBM patients. Cerebral infarction can occur in both the acute stage and later stages of treatment. Mortality and morbidity are high, and early diagnosis and appropriate antimicrobial treatment are essential. If hydrocephalus is demonstrated, early ventricular decompression is needed to prevent further cerebral ischaemia.

 

2864.    Limcangco MR.  Armour CL.  Salole EG.  Taylor SJ. Cost-benefit analysis of a Haemophilus influenzae type b meningitis prevention programme in The Philippines. [Review] [49 refs] Pharmacoeconomics.  19(4):391-400, 2001.

Abstract

  BACKGROUND: Haemophilus influenzae type b (Hib) meningitis is associated with high mortality and serious sequelae in children under 5 years of age. Vaccines which can prevent this infection are available. OBJECTIVE: To evaluate the costs and benefits of a 3-dose immunisation schedule in Manila, Philippines. PERSPECTIVE: Government and societal perspectives. DESIGN AND PARTICIPANTS: A cost-benefit analysis based on a birth cohort of 100,000 children. The state of health of the cohort with and without a Hib immunisation programme was modelled over a 5-year period. A survey of medical records of patients with Hib in Manila provided data on the extent and cost of sequelae following infection. INTERVENTION: A 3-dose Hib vaccination programme given at ages 2, 3 and 4 months. RESULTS: The model predicted that vaccinating children against Hib meningitis would prevent 553 cases per year in a birth cohort of 100,000, at a cost of 56,200 Philippine pesos (PHP) [$US1,605; 1998 exchange rate] per case (base case assumptions of 90% vaccine efficacy rate, 95 per 100,000 Hib incidence rate, 85% vaccination coverage). Results from the cost-benefit analyses indicated that the saving to the government would be around PHP39 million ($US1.11 million), and the saving to society would be PHP255 million ($US7.28 million). CONCLUSION: There would be a positive economic benefit for the Philippine government and for the Filipino society if a Hib vaccination programme was introduced in Manila. [References: 49]

 

2865.    MacLean LL.  Vinogradov E.  Crump EM.  Perry MB.  Kay WW. The structure of the lipopolysaccharide O-antigen produced by Flavobacterium psychrophilum (259-93). European Journal of Biochemistry.  268(9):2710-6, 2001 May.

Abstract

  Flavobacterium psychrophilum, a Gram-negative bacterium, is the etiological agent of rainbow trout fry syndrome and bacterial cold water disease, septicemic infections in reared salmonids. In humans Flavobacterium spp. have been associated with neonatal meningitis and septicemia, catheter-associated bacteremia, and pneumonia. Recently, several F. psychrophilum surface molecules, including lipopolysaccharide (LPS), have been implicated in its pathogenesis and identified as potential vaccine and diagnostic candidate macromolecules. Studies on the LPS produced by the bacterium are reported herein. The structure of the antigenic O-polysaccharide contained in the LPS of F. psychrophilum was deduced by the application of analytical NMR spectroscopy, mass spectrometry, glycose and methylation analysis, and partial hydrolysis degradations, and was found to be an unbranched polymer of trisaccharide repeating units composed of L-rhamnose (L-Rhap), 2-acetamido-2-deoxy-L-fucose (L-FucpNAc) and 2-acetamido-4-((3S,5S)-3,5-dihydroxyhexanamido)-2,4-dideoxy-D-quinovose (D-Quip2NAc4NR, 2-N-acetyl-4-N-((3S,5S)-3,5-dihydroxyhexanoyl)-D-bacillosamine) (1 : 1 : 1) and having the structure: -->4)-alpha-L-FucpNAc-(1-->3)-alpha-D-Quip2NAc4NR-(1-->2)- alpha-L-Rhap-(1--> where R is (3S,5S)-CH3CH(OH)CH2CH(OH)CH2CO-.

 

2866.    Maclennan J.  Meningococcal group C conjugate vaccines. Archives of Disease in Childhood.  84(5):383-6, 2001 May.

 

2867.    Marcos MA.  Martinez E.  Almela M.  Mensa J.  Jimenez de Anta MT. New rapid antigen test for diagnosis of pneumococcal meningitis. Lancet.  357(9267):1499-500, 2001 May 12.

Abstract

  Conventional diagnostic methods for bacterial meningitis are frequently not rapid or sensitive enough to guide initial antimicrobial therapy. Streptococcus pneumoniae is the most frequent and severe cause of community-acquired bacterial meningitis and treatment is complicated by the increasing prevalence of antimicrobial resistance to third-generation cephalosporins. We used a new rapid antigen test in the cerebrospinal fluid and urine of patients with suspected bacterial meningitis, and found it to be highly sensitive and specific for the detection of pneumococci. This test might help guide initial therapy for bacterial meningitis according to the local rates of pneumococcal antimicrobial resistance.

 

2868.    Massung RF.  Davis LE.  Slater K.  McKechnie DB.  Puerzer M. Epidemic typhus meningitis in the southwestern United States.  Clinical Infectious Diseases.  32(6):979-82, 2001 Mar 15.

Abstract

  A patient residing in New Mexico had murine typhus diagnosed. A novel molecular assay was performed at the Centers for Disease Control and Prevention, and Rickettsia prowazekii, the agent of epidemic typhus, was found, rather than R. typhi. To our knowledge, this is the first reported case of epidemic typhus confirmed by means of polymerase chain reaction--based testing of cerebrospinal fluid, and it introduces a novel assay for the molecular diagnosis of both epidemic and murine typhus.

 

2869.    McIntire SC.  Green M.  Fever and stiff neck.  Archives of Pediatrics & Adolescent Medicine.  155(5):603-6, 2001 May.

 

2870.    Narchi H.  Aseptic meningitis.  Pediatrics.  107(2):451, 2001 Feb.

 

2871.    Nash D.  Mostashari F.  Fine A.  Miller J.  O'Leary D.  Murray K.  Huang A.  Rosenberg A.  Greenberg A.  Sherman M.  Wong S.  Layton M.  1999 West Nile Outbreak Response Working Group.  The outbreak of West Nile virus infection in the New York City area in 1999. [see comments]. New England Journal of Medicine.  344(24):1807-14, 2001 Jun 14.

Abstract

  BACKGROUND: In late August 1999, an unusual cluster of cases of meningoencephalitis associated with muscle weakness was reported to the New York City Department of Health. The initial epidemiologic and environmental investigations suggested an arboviral cause. METHODS: Active surveillance was implemented to identify patients hospitalized with viral encephalitis and meningitis. Cerebrospinal fluid, serum, and tissue specimens from patients with suspected cases underwent serologic and viral testing for evidence of arboviral infection. RESULTS: Outbreak surveillance identified 59 patients who were hospitalized with West Nile virus infection in the New York City area during August and September of 1999. The median age of these patients was 71 years (range, 5 to 95). The overall attack rate of clinical West Nile virus infection was at least 6.5 cases per million population, and it increased sharply with age. Most of the patients (63 percent) had clinical signs of encephalitis; seven patients died (12 percent). Muscle weakness was documented in 27 percent of the patients and flaccid paralysis in 10 percent; in all of the latter, nerve conduction studies indicated an axonal polyneuropathy in 14 percent. An age of 75 years or older was an independent risk factor for death (relative risk adjusted for the presence or absence of diabetes mellitus, 8.5; 95 percent confidence interval, 1.2 to 59.1), as was the presence of diabetes mellitus (age-adjusted relative risk, 5.1; 95 percent confidence interval, 1.5 to 17.3). CONCLUSIONS: This outbreak of West Nile meningoencephalitis in the New York City metropolitan area represents the first time this virus has been detected in the Western Hemisphere. Given the subsequent rapid spread of the virus, physicians along the eastern seaboard of the United States should consider West Nile virus infection in the differential diagnosis of encephalitis and viral meningitis during the summer months, especially in older patients and in those with muscle weakness.

 

2872.    Reiber H.  Peter JB.  Cerebrospinal fluid analysis: disease-related data patterns and evaluation programs. [Review] [75 refs] Journal of the Neurological Sciences.  184(2):101-22, 2001 Mar 1.

Abstract

  Cerebrospinal fluid (CSF) analysis is a basic tool for diagnosis of neurological diseases. Knowledge regarding blood-CSF barrier function (molecular flux/CSF flow theory) and neuroimmunology is reviewed to aid understanding and evaluation of CSF data. Disease-related immunoglobulin patterns (IgG, IgA, IgM with reference to albumin) are described in CSF/serum quotient diagrams with the hyperbolic reference range for blood-derived protein fractions in CSF. Clinical relevance of complementary analyses (cytology, PCR, oligoclonal IgG, antibody detection and brain-derived proteins) is briefly discussed. Integrated CSF data reports are shown with numerical and graphical data representation, reference range-related interpretation and diagnosis-related comments. The principles and rationale of general CSF analysis reported in this review should enable the reader to accurately interpret CSF data profiles, and to plan a proper evaluation of new brain- or blood-derived analytes in CSF. [References: 75]

 

2873.    Salleras L.  Dominguez A.  Prats G.  Parron I.  Munoz P. Dramatic decline of serogroup C meningococcal disease incidence in Catalonia (Spain) 24 months after a mass vaccination programme of children and young people.  Journal of Epidemiology & Community Health.  55(4):283-7, 2001 Apr.

Abstract

  STUDY OBJECTIVES: The objective of this study was to evaluate the effectiveness of a mass vaccination programme carried out in Catalonia (Spain) in the last quarter of 1997 in response to an upsurge of serogroup C meningococcal disease (SCMD). DESIGN: Vaccination coverage in the 18 month to 19 years age group was investigated by means of a specific vaccination register. Vaccination effectiveness was calculated using the prospective cohort method. Cases of SCMD were identified on the basis of compulsory reporting and microbiological notification by hospital laboratories. Vaccination histories were investigated in all cases. Unadjusted and age adjusted vaccination effectiveness referred to the time of vaccination and the corresponding 95% confidence intervals (CI) were estimated at 6, 12, 18 and 24 months of follow up. SETTING: All population aged 18 months to 19 years of Catalonia. MAIN RESULTS: A total of seven cases of SCMD were detected at six months of follow up (one in the vaccinated cohort), 12 cases at 12 months (one in the vaccinated cohort), 19 cases at 18 months (two in the vaccinated cohort) and 24 at 24 months (two in the vaccinated cohort). The age adjusted effectiveness was 84% (95%CI 30, 97) at six months, 92% (95%CI 63, 98) at 12 months, 92% (95% CI 71, 98) at 18 months and 94% (95%CI 78, 98) at 24 months. In the target population, cases have been reduced by more than two thirds (68%) two years after the vaccination programme. In the total population the reduction was 43%. CONCLUSION: Vaccination effectiveness has been high in Catalonia, with a dramatic reduction in disease incidence in the vaccinated cohort accompanied by a relevant reduction in the overall population. Given that vaccination coverage was only 54.6%, it may be supposed that this vaccination effectiveness is attributable, in part, to the herd immunity conferred by the vaccine.

 

2874.    Somali MH.  Anastasiou AL.  Goulis DG.  Polyzoides C.  Avramides A. Pituitary abscess presenting with cranial nerve paresis. Case report and review of literature. [Review] [45 refs]  Journal of Endocrinological Investigation.  24(1):45-50, 2001 Jan.

Abstract

  Non-adenomatosus lesions of the pituitary represent a small part of the intrasellar processes and they have heterogeneous presentation. Making a precise diagnosis is of great importance, as it may lead to more efficient management. A 65-year-old man was admitted to the hospital because of headache and right cranial nerve III palsy. Basic laboratory work-up was normal whereas endocrinological assessment revealed hypopituitarism without diabetes insipidus. Plain radiography showed an enlarged sella and frontal and paranasal sinusitis. Computed tomography (CT) and magnetic resonance imaging (MRI) of the sella revealed an intrasellar lesion with extension to the sphenoid and cavernous sinuses as well as the suprasellar region, exerting pressure on the optic chiasm. On T1-weighted images the mass had a low-intensity signal with a smooth enhancing rim with bright signal. Given the presence of multiple sinusitis and imaging characteristics a pre-operative diagnosis of pituitary abscess was made. The patient was operated via transphenoidal route and purulent material was drained out. Cultures of the material were positive for Staphylococcus aureus. Antibiotics as well as cortisol replacement therapy were given. Three months later hypopituitarism persisted but there was significant improvement in the neurological findings. We report a case of an unusual presentation of a pituitary abscess. High index of suspicion, the presence of associated conditions such as pituitary tumors, meningitis or sinusitis, as well as diabetes insipidus and specific imaging features are the main diagnostic clues. Pre-operative diagnosis, which will lead to prompt antibiotic therapy and transphenoidal drainage, can decrease high mortality and morbidity associated with this disease. [References: 45]

 

 

2875.    Tsui EY.  Loo KT.  Mok CK.  Yuen MK.  Cheung YK. Primary multifocal leptomeningeal gliomatosis.  European Journal of Radiology.  37(1):5-7, 2001 Jan.

Abstract

  A 23-year-old female university student was presented with recent onset of non-specific headache and dizziness. She had no neurological deficit on neurological examination and magnetic resonance imaging of the brain revealed diffuse enhancement in the basal cisterns and cerebral sulci. She was treated as tuberculous meningitis but she did not improve and developed respiratory arrest. Autopsy showed primary multifocal leptomeningeal gliomatosis.

 

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