AMOEBIASIS  

Some Selected Abstracts:

1.

Roy S, Kabir M, Mondal D, Ali IK, Petri WA Jr, Haque R. Real-time-PCR assay for diagnosis of Entamoeba histolytica infection. J Clin Microbiol. 2005 May;43(5):2168-72.

International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,b), Dhaka, Bangladesh.

We developed a real-time-PCR assay utilizing a molecular-beacon probe for the detection of Entamoeba histolytica and compared its sensitivity to stool antigen detection and traditional PCR. A total of 205 stool and liver abscess pus specimens from patients and controls were used for this purpose, 101 (49%) of which were positive by the TechLab E. histolytica-specific antigen detection test, while the other 104 (51%) stool and liver abscess pus specimens were negative by the antigen detection test. DNA was extracted from the stool and liver abscess pus specimens by the QIAGEN method and the small-subunit rRNA gene of E. histolytica and then amplified by traditional and real-time PCR. Out of these 205 stool and liver abscess pus specimens, 124 were positive by the real-time-PCR assay and 90 were positive by the traditional-PCR test. Compared to the real-time-PCR assay, the antigen detection test was 79% sensitive and 96% specific. When the traditional-PCR test results were compared to the real-time-PCR assay, the sensitivity of traditional PCR was 72% and the specificity was 99%. In conclusion, all three methods for the detection of E. histolytica were highly specific, with real-time PCR being the most sensitive.

Diagnostics, Immunodiagnosis & Immunodiagnostics:  

12517.  Roy S, Kabir M, Mondal D, Ali IK, Petri WA Jr, Haque R. Real-time-PCR assay for diagnosis of Entamoeba histolytica infection. J Clin Microbiol. 2005 May;43(5):2168-72
Pathogenesis:

12517.    Kaji Y, Hu B, Kawana K, Oshika T. Swimming with soft contact lenses: danger of acanthamoeba keratitis. Lancet Infect Dis. 2005 Jun;5(6):392.

12518.  Pinheiro SM, Maciel RF, Morais MA Jr, Aca IS, Carvalho LB Jr, Coimbra MR. Genetic characterization of Entamoeba dispar isolates in Northeast Brazil. Acta Trop. 2005 Apr;94(1):35-40. 

12519.  Sim S, Yong TS, Park SJ, Im KI, Kong Y, Ryu JS, Min DY, Shin MH. NADPH oxidase-derived reactive oxygen species-mediated activation of ERK1/2 is required for apoptosis of human neutrophils induced by Entamoeba histolytica. J Immunol. 2005 Apr 1;174(7):4279-88.

12520. Sissons J, Kim KS, Stins M, Jayasekera S, Alsam S, Khan NA. Acanthamoeba castellanii induces host cell death via a phosphatidylinositol 3-kinase-dependent mechanism. Infect Immun. 2005 May;73(5):2704-8.

Therapy:

12521.  Nazir Z, Qazi SH. Amebic liver abscesses among neonates can mimic bacterial sepsis. Pediatr Infect Dis J. 2005 May;24(5):464-6. 

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ASCARIASIS

Diagnosis, Diagnostics, Immunodiagnosis & Immunodiagnostics:

12523.  Sherman SC, Weber JM.  The CT diagnosis of Ascariasis. J Emerg Med. 2005 May;28(4):471-2.
Pathogenesis:

12524.  Faulkner H, Turner J, Behnke J, Kamgno J, Rowlinson MC, Bradley JE, Boussinesq M.  Associations between filarial and gastrointestinal nematodes. Trans R Soc Trop Med Hyg. 2005 Apr;99(4):301-12.

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