Typhoid

Selected abstract:

1.                  Hatta M, Smits HL. Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples. Am J Trop Med Hyg. 2007 Jan;76(1):139-43.

Department of Medical Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia.

A nested polymerase chain reaction (PCR) specific for Salmonella enterica serovar Typhi was used for the detection of the pathogen in blood, urine, and stool samples from 131 patients with clinical suspicion of typhoid fever. The sensitivity of blood culture, the PCRs with blood, urine, and feces, and the Widal test were 61.8%, 84.5%, 69.3%, 46.9%, and 39.0%, respectively. The sensitivity of the PCRs with blood (P < 0.001) and urine (P = 0.01) were significantly higher, and the sensitivity of the PCR with feces (P > 0.05) was similar to that of blood culture. Combined, the PCRs on urine and feces showed positive results for 16 (70%) of 23 typhoid patients with negative results with blood culture and PCR with blood. These results show that the PCR with blood is a sensitive method for the diagnosis of typhoid fever, and that the PCRs with urine and feces could be useful complementary tests.

Diagnosis, Diagnostics, Immunodiagnosis & Immunodiagnostics:

16114.  Kawano RL, Leano SA, Agdamag DM. Comparison of serological test kits for diagnosis of typhoid fever in the Philippines. J Clin Microbiol. 2007 Jan;45(1):246-7.

16115.  Mantur BG, Bidari LH, Akki AS, Mulimani MS, Tikare NV. Diagnostic yield of blood clot culture in the accurate diagnosis of enteric fever and human brucellosis. Clin Lab. 2007;53(1-2):57-61.

Vaccines:

16116.  Kaljee LM, Pham V, Son ND, Hoa NT, Thiem VD, Canh do G, Thoa le TK, Ali M, Ochiai RL, Danovaro-Holliday MC, Acosta CJ, Stanton B, Clemens J. Trial participation and vaccine desirability for Vi polysaccharide typhoid fever vaccine in Hue City, Viet Nam. Trop Med Int Health. 2007 Jan;12(1):25-36.

Chemotherapy, Immunotherapy, Management & Drugs:

16117.  Toovey S, Moerman F, van Gompel A. Special infectious disease risks of expatriates and long-term travelers in tropical countries. Part II: infections other than malaria. J Travel Med. 2007 Jan-Feb;14(1):50-60. Review.