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Dabir S, Dabir P, Reddy MVR. Isolation and characterization of a major form of superoxide dismutase from human lymphatic filarial parasite, brugia malayi. Biomedical Research, 2005; 16(1): 19-22.

Superoxide dismutase (SOD) is an enzyme that converts superoxide radicals into hydrogen peroxide and molecular oxygen and provides first line of defense against oxygen toxicity. We have isolated and characterized the extracellular form of superoxide dismutase from lymphatic filarial nematode parasite Brugia malayi. The enzyme activity was higher in adult worms (30.29 U/mg) than in microfilariae (23.19 U/mg). The parasite extracts were also analyzed by native polyacrylamide gel electrophoresis on 12% gel fallowed by staining for enzyme SOD activity using nitroblue tetrazolium (NBT), N, N, N, N tetra methyl ethylene diamine (TEMED) and riboflavin. A very broad band of enzyme activity was observed in both mf and adult worms extracts, with the latter showing more intense band. Fractionation of adult worm extracts by Fast Performance Liquid Chromatography (FPLC) using superdex 75 HR 10/30 column also showed polydispersed nature of SOD activity with 10 of the 15 fractions having varying levels of enzyme activity. The 9^th fraction with highest SOD activity was found to be a 29 kDa molecule having cross reactivity with SOD of B. pahangi. Further it was characterized as CuZn SOD based on significant inhibition of its activity by potassium cyanide.

Diagnosis, Diagnostics, Immunodiagnosis & Immunodiagnostics:

11416.     Dabir S, Dabir P, Reddy MVR. Isolation and characterization of a major form of superoxide dismutase from human lymphatic filarial parasite, brugia malayi. Biomedical Research, 2005; 16(1): 19-22.

11417.     Hussein O, Setouhy ME, Ahmed ES, Kandil AM, Ramzy RM, Helmy H, Weil GJ. Duplex Doppler sonographic assessment of the effects of diethylcarbamazine and albendazole therapy on adult filarial worms and adjacent host tissues in Bancroftian filariasis. Am J Trop Med Hyg. 2004 Oct;71(4):471-7.

11418.     Oshiro Y, Murayama S, Sunagawa U, Nakamoto A, Owan I, Kuba M, Uehara T, Miyahira T, Kawabata T, Kuniyoshi M, Ishikawa K, Kinjyo T, Fujimoto K, Yamada K. Pulmonary dirofilariasis: computed tomography findings and correlation with pathologic features.J Comput Assist Tomogr. 2004 Nov-Dec;28(6):796-800.

11419.  Simonsen PE, Magesa SM. Observations on false positive reactions in the rapid NOW Filariasis card test. Trop Med Int Health. 2004 Nov;9(11):1200-2.

Pathogenesis:

11420.    Diaz LA, Arteaga LA, Hilario-Vargas J, Valenzuela JG, Li N, Warren S, Aoki V, Hans-Filho G, Eaton D, dos Santos V, Nutman TB, de Mayolo AA, Qaqish BF, Sampaio SA, Rivitti EA; Cooperative Group on Fogo Selvagem Research.  Anti-desmoglein-1 antibodies in onchocerciasis, leishmaniasis and Chagas disease suggest a possible etiological link to Fogo selvagem. J Invest Dermatol. 2004 Dec;123(6):1045-51.

11421.  Specht S, Volkmann L, Wynn T, Hoerauf A.  Interleukin-10 (IL-10) counterregulates IL-4-dependent effector mechanisms in Murine Filariasis. Infect Immun. 2004 Nov;72(11):6287-93.