Facilities at JBTDRC & Department of Biochemistry
Molecular Biology Laboratory
Cell Culture Laboratories
PG Research Laboratory
The centre has acquired sophisticated instruments such as FPLC – System (Pharmacia), Freeze drier (Virtis), ELISA Reader (ERBA), Cryopreservation equipment (Forma scientific), Thermocycler (PCR) equipment (Biometra), CO2 Incubator (Forma Scientific), Orbital-Shaker (Forma Scientific), Ultracentrifuge (Kontron), Spectrophotometer (Gilford), Minicold Lab with Chromatography equipment, ELISA Reader (DynaTech)., Walk in cold room, Gamma Isotope Counters, SDS-PAGE & Sub-marine multiphoresis electrophoresis apparatus, -700C Freezers (Forma & Revco) and Millipore Biocel Water Purification System, Gel-Doc System (Kodak), Pellicone Ultrafiltration System (Millipore) and Liquid Scintillation Counter (Packard) for biomedical research. In addition the centre has National Repository for filarial parasites supported by DBT, insectarium and animal house with filarial animal models. B.malayi is being maintained in rodents (Swiss and Desert rats), the second institute in India.
Filaria Animal Model
A National Repository facility for filarial parasites and reagents is set up with the support from DBT. Brugia malayi filarial infection susceptible in rodents mastomys and jirds ( second facility in India) and a filarial sera bank with a collection of about 300 sera of different clinical groups of bancroftian filariasis from various endemic regions are being maintained.
Meriones unguiculatus (Jird)
Mastomys natalensis (Swiss rats)
There is an independent CPCSEA registered animal house of about 1800 sq. ft area (constructed with support of TDR and DBT grants) housing about 140 Mastomys natalensis (Swiss rats) & 220 Meriones unguiculatus (Desert rats) as filarial animal models and 10 Balb/c mice (for hybridoma technique) and 12 rabbits for raising antisera.
This is a breeding and rearing facility for Aedes aegypti (Liverpool black eye strain) mosquito colony to be used as vector for transmission of B. malayi infection to Mastomys & jirds.